螺旋藻工厂化培养条件优化及营养成分比较  

Culture Condition and Nutritional Components of Spirulina platensis in Spring under Industrialized Condition

作　　者：常林[1] 邹宁[1] 孙东红[1] 杜晓凤[1] 

机构地区：[1]鲁东大学,山东烟台264025

出　　处：《安徽农业科学》2012年第9期5140-5142,5151,共4页Journal of Anhui Agricultural Sciences

基　　金：山东高校科技计划-自制生物反应器自动监控系统的研究(J05103)

摘　　要：[目的]对螺旋藻工厂化的培养条件进行优化,并对其营养成分的含量进行比较。[方法]在以采光板作顶棚的封闭式车间内,采用4种不同直径的光生物反应器对春季螺旋藻进行培养,并对不同直径的光生物反应器所能达到的最大细胞密度及各种主要营养成分的含量进行比较。[结果]在20 cm的光生物反应器所能达到的最大细胞密度最大,为1.52 g/L。20 cm的光生物反应器培养的螺旋藻所含蛋白质和藻蓝蛋白含量最高;蛋白质含量最高可以达到61.2%,藻蓝蛋白含量最高可以达到10.9%。10 cm的光生物反应器培养的螺旋藻所含的粗脂肪、多糖和叶绿素a的含量最高、粗脂肪的含量最高可以达到7.48%,多糖最高可以达到8.2%,叶绿素a可以达到1.3 mg/g。[结论]该方法优化了螺旋藻工厂化的培养条件,为螺旋藻的开发利用提供了理论依据。[ Objective ] To optimize the culture condition of Spirulina platensis under industrialized condition and to compare the contents of nutritional components in spring. [ Method ] In the enclosed workshop with collecting light panel as the ceiling, S. platensis was cultured by photo bioreactors with four different optical paths. The maximum cell densities of photo bioreactors with different optical paths were compared as well as the contents of different nutritional components. [ Result] The maximal cell density was 1.52 g/L under 20 cm light - path photo biore- actor. The contents of protein and phycocyanin were the highest in 20 cm light path ,which were 61.2% and 10.9% , respectively. The contents of carbohydrate, curde fat and chlorophyll a were the highest in 10 cm light path, the content of curde fat reached 7.48% , the content of carbo- hydrate reached 8.2% ,and the content of chlorophyll a reached 1.3 mg/g. [ Conclusion] This method optimized the culture condition of Spirulina platensis under industrialized condition,which provided theoretical references for the development and utilization of S. platensis.

关 键 词：螺旋藻 光生物反应器 细胞密度 营养成分 

分 类 号：S968.4[农业科学—水产养殖]