菊芋生料联合生物加工发酵生产燃料乙醇  

Production of Fuel Ethanol from Uncooked Helianthus tuberosus by Consolidated Bio-processing(CBP) Fermentation

作　　者：杨梅[1] 袁文杰[2] 凤丽华[3] 

机构地区：[1]吉林化工学院环境与生物工程学院,吉林吉林132022 [2]大连理工大学生物科学与技术学院,辽宁大连116023 [3]中国石油吉林石化公司炼油厂,吉林吉林132022

出　　处：《安徽农业科学》2012年第9期5438-5441,共4页Journal of Anhui Agricultural Sciences

基　　金：辽宁省基金项目(2008RC57)

摘　　要：[目的]在联合生物加工技术发酵菊芋生料生成乙醇工艺中,降低高浓度菊芋生料醪液的黏度,使菊芋发酵乙醇浓度达到或超过目前玉米乙醇的行业水平。[方法]利用马克斯克鲁维酵母(Kluyveromyces marxianus),通过添加辅助酶制剂、改变拌料水温及优化补料方式等方法,降低发酵醪液黏度,提高菊芋生料的浓度,从而提高发酵终点的乙醇浓度并缩短发酵时间。[结果]最佳工艺条件为:45℃水拌料,pH 5.0,添加0.10%酒精复合酶,发酵初始干粉浓度为240 g/L,分别在发酵12和24 h补料,菊芋干粉终浓度可达到300 g/L,发酵时间48 h,乙醇浓度达到91.6 g/L,乙醇对糖的得率为0.464,为理论值的90.6%。[结论]此工艺为菊芋乙醇工业化的生产提供了有利条件。[ Objective] During the process of producing ethanol from uncooked Helianthus tuberosus by consolidated bio-processing （CBP）fermentation, the viscosity of high-concentration uncooked Helianthus tuberosus wort was decreased, which could reach or exceed the current industry level of eorn ethanol. [ Method ] By using Kluyveromyees marxianus, through adding assistant enzyme, changing water temperature and optimizing fed-batch method, the wort viscosity was decreased and concentration of uncooked Helianthus tuberosus was improved, andthen, the final fermentation ethanol concentration was increased and fermentation time was reduced. [ Result] The optimum processing conditions were ： temperature of water 45℃ , initial pH 5.0, adding 0.10% alcohol compound enzymes, fed-hatched at 12 h and 24 h, initial concentration of dry powder was 240 g/L, the final concentration reached 300 g/L, the final ethanol concentration was 91.6 g/L and ethanol yield was obtained 0.464 for the theoretical value of 90.6% at 48 h. [ Conclusion ] The process will provide favorable conditions for industrialization production of Helianthus tuberosus ethanol.

关 键 词：菊芋 燃料乙醇 联合生物加工 马克斯克鲁维酵母 批式补料 

分 类 号：S632.9[农业科学—蔬菜学]