氨三乙酸对废水厌氧消化过程中产甲烷的影响  

Effect of nitrilotriacetic acid on methane production during anaerobic digestion of wastewaters

作　　者：陈文宾[1,2] 胡庆昊[1] 韩晶晶[2] 徐国想[1] 祝春水[1,2] 邓宇[3] 

机构地区：[1]淮海工学院化学工程学院,江苏连云港222005 [2]中国矿业大学环境与测绘工程学院,江苏徐州221116 [3]农业部能源微生物与利用重点开放实验室,四川成都610041

出　　处：《中国矿业大学学报》2012年第2期334-338,共5页Journal of China University of Mining & Technology

基　　金：江苏省自然科学基金项目(BK2009652);连云港市社会发展计划项目(SH0921);农业部能源微生物与利用重点开放实验室开放基金项目(004)

摘　　要：在连续运行的槽式搅拌反应器(CSTR)中考察了氨三乙酸(NTA)对厌氧消化反应器运行效果的影响.在中温35℃条件下,有机负荷化学需氧量(COD)为1.0～3.4g/(L.d)时,NTA的添加可使CSTR反应器COD去除率增加,产气率提高20%～42%,反应器中厌氧污泥的辅酶F430和F420浓度分别提高了1.33倍和24%;采用荧光原位杂交技术(FISH)对古细菌和甲烷八叠球菌进行了检测,结果表明:NTA还促进了甲烷八叠球菌的生长.作为微量元素螯合剂,NTA增强了微量元素镍的生物可利用性,优化了产甲烷菌的营养条件,强化了产甲烷菌对微量元素的生物吸收,对厌氧消化反应具有促进作用.Abstract： The effect of nitrilotriacetic acid（NTA） on anaerobic digestion in a continuous stirred tank reactor（CSTR） was investigated. At 35 ~C and organic loading COD rate of 1.0--3.4 g/ （L · d）, the COD removal efficiency was improved and biogas yields were increased by 20%-- 42% by addition of NTA. The contents of coenzyme F430 and F420 in the anaerobic sludge were increased by 1.33 times and 24%, respectively. The archaebacteria and methanosarcinaeeae were detected by a fluorescence in situ hybridization（FISH）. The results show that NTA stim- ulate the growth of Methanosarcinaceae. Due to the addition of NTA, a trace metal chelating agent, the bioavailability of trace metal such as nickel is increased and the nutrient condition is optimized for methane production bacteria（MPB）. The biouptake of trace metal for MPB is strengthened. NTA has stimulative effect on anaerobic digestion.

关 键 词：氨三乙酸 厌氧消化 产甲烷菌 螯合剂 

分 类 号：X172[环境科学与工程—环境科学]