补体C3对神经病理性疼痛模型脊髓星形胶质细胞的影响  

EFFECTS OF COMPLEMENT 3 ON ASTROCYTE ACTIVATION IN THE NEUROPATHIC PAIN MODEL MICE

作　　者：徐涛[1] 魏安宁[1] 黄良库[1] 聂发传[1] 

机构地区：[1]重庆医科大学附属二院麻醉科,渝中400016

出　　处：《中国疼痛医学杂志》2012年第3期169-173,共5页Chinese Journal of Pain Medicine

基　　金：国家自然科学基金项目(基金编号30772077)

摘　　要：目的:观察补体C3对神经病理性疼痛模型脊髓星形胶质细胞的影响。方法:81只补体C3基因敲除小鼠随机分三组(n=27):A组:假手术组;B组:慢性坐骨神经结扎模型(CCI)组;C组:CCI模型补体C3干预组。测定小鼠的热痛阈值和机械痛阈值,并取腰5、6脊髓节段测定GFAP mRNA和GFAP表达。结果 :术前三个组小鼠热和机械痛阈无明显差异,术后1天A组热和机械痛阈下降,其后恢复。B组和C组继续下降,C组下降幅度更大(P<0.05)。术后第1天,B组和C组胶质细胞激活轻微,术后第3、7天B组和C组脊髓组织GFAP mRNA和GFAP表达量逐渐增加,且C组其表达量明显高于B组。结论 :补体C3的存在与神经病理性模型小鼠星形胶质细胞激活显著相关,并由此影响到慢性疼痛状态的出现和维持。Objective： To observe the effects of complement 3 on astrocyte activation in neuropathic pain （NPP） mice. Methods： 81 C3-gene knock out mice were divided randomly into 3 groups as following （n = 27 in each group）： Group A received sham operation; Group B received chronic constriction injury （CCI） operation; Group C received CCI operation and complement 3 lateral ventricle injection postoperation. Pain threshold by thermal or mechanical stimulation were recorded respectively. The levels of GFAP and the expression of GFAP mRNA in Ls, 6 spinal cord were detected. Results： Pain threshold of paw withdrawal decreased at the 1st day postoperation in all mice. The decreased threshold recovered fleetly in Group A. Those in Group B and Group C significantly began to decrease in pain threshold of paw withdrawal. And those in Group C showed more magnitude of changing range. A slight degree of astrocytic activation was observed in B and C group at 1 day after operation. GFAP and GFAP mRNA expression began to increase 3 d, 7 d after operation in B and C group. GFAP and GFAP mRNA in Group C was obviously more than those in Group B. Conclusion： The existence of complement C3 was significantly related to astrocyte activation in the neuropathic pain model mice, and involved in the initiation and maintaining of neuropathic pain.

关 键 词：补体蛋白 神经病理性疼痛 基因敲除小鼠 星形胶质细胞 

分 类 号：R741[医药卫生—神经病学与精神病学]