胃液对膀胱癌细胞系Bcl-2Bax表达的影响  

Effect of Gastric Juice on Bcl-2/Bax Expression of Human Bladder Cancer Cell Line

作　　者：田斌群[1] 胡礼泉[1] 郑新民[1] 姚学军[2] 李世文[1] 王家治[1] 张国辉[1] 

机构地区：[1]湖北医科大学附属第二医院泌尿外科,武汉430071 [2]湖北医科大学病毒研究所

出　　处：《肿瘤防治研究》2000年第1期43-45,共3页Cancer Research on Prevention and Treatment

基　　金：湖北省教委资助!(G - 91- 12 )

摘　　要：目的　为了探讨胃液诱导膀胱癌细胞系细胞凋亡的机制。方法　采用流式细胞术及免疫组化方法观察了胃液对膀胱癌细胞系BIU 87细胞凋亡及Bcl 2 Bax表达的影响。结果　显示胃液处理后AI值升高 ,Bcl 2表达降低 ,Bax表达升高 ,Bcl 2 Bax比值为 0 71。结论　提示胃液诱导BIU 87细胞凋亡可能是通过降低Bcl 2 ,升高Bax而实现的。Objective To study the mechanism of apoptosis induced by gastric juice.Methods Flow cytometry and immunohistochemistry were utilized to observe the effect of gastric juice on Bcl 2/Bax expression of human bladder cancer cell line BIU 87.Results After administration of gastric juice,AI increased,Bcl 2 expression reduce,Bax expression increased and Bcl 2/Bax ratio was 0 71.Conclusion It was suggested that gastric juice could induce the apoptosis of BIU 87 by reducing Bcl 2 and increasing Bax.

关 键 词：胃液 膀胱癌细胞系 癌细胞凋亡 BCL-2/BAX 

分 类 号：R737.14[医药卫生—肿瘤]