选择性水解工艺提取蔗髓L-阿拉伯糖的研究  

Study on Extraction of L-arabinose from Bagasse Pith by Hot Water Selective Hydrolysis Process

作　　者：李娜[1] 刘秋娟[1] 江超[1] 

机构地区：[1]天津科技大学天津市制浆造纸重点实验室,天津300457

出　　处：《造纸科学与技术》2012年第1期30-33,共4页Paper Science & Technology

摘　　要：用热水处理蔗髓,通过控制水解温度使L-阿拉伯糖选择性水解以利于L-阿拉伯糖的提取。考察了温度、水解时间及液比对蔗髓水解产出L-阿拉伯糖、木糖及葡萄糖的影响。采用高效液相色谱法对水解液中的单糖含量进行了检测,结果表明:温度对水解液组分的影响最为显著,当温度较低(≤140℃)时,L-阿拉伯糖有明显溶出,而木糖与葡萄糖溶出很少甚至不溶出;当温度超过160℃,木糖产率大于L-阿拉伯糖与葡萄糖;在较低温度下延长保温时间能够使L-阿拉伯糖有较大量溶出,同时木糖及葡萄糖溶出很少。实验得到蔗髓水解提取L-阿拉伯糖的适宜条件为:水解温度140℃,保温时间3h,液比1∶10,此时L-阿拉伯糖的产率为1.40%,木糖、葡萄糖未检出。Bagasse pith was treated with hot water in this study. By controlling the hydrolysis temperature, L-arabi- nose was selectively hydrolyzed from bagasse pith, which was favorable for extraction and separation. The effect of different temperature, time and liquor ratio on concentration of L-arabinose, xylose and glucose of the hydrolyzate were discussed. Quantitative analysis of L-arabinose, xylose and glucose was conducted by HPLC. Results indica- ted that temperature was the most important factor which affected the composition of the hydrolyzate. L-arabinose was markedly extracted while xylose and glucose were hardly detected at a lower temperature（ ≤ 140°C ）. When the temperature was higher than 160°C, more xylose was extracted than L-arabinose and glucose. A lower temperature and a longer holding time was favorable for extraction of L-arabinose, under which no measurable quantities of xylose and glucose were produced. The yield of L-arabinose reached 1.40% at the maximum temperature of 140°C , the holding time of 3h, the liquor ratio of 1 ： 10, with undetected xylose and glucose.

关 键 词：蔗髓 L-阿拉伯糖 选择性水解 

分 类 号：Q532[生物学—生物化学]