AFM研究蝙蝠葛碱对daudi细胞毒性的影响  

Study of Cell Toxicity of Dauricine on Daudi Cells Based on Atomic Force Microscope

作　　者：陈伟[1,2] 王穗湘[3] 胡小毛[4] 刑晓波[1,2] 蔡继业[1,2] 

机构地区：[1]暨南大学光电信息与传感技术广东普通高校重点实验室,广东广州510632 [2]暨南大学化学系,广东广州510632 [3]暨南大学医学院,广东广州510632 [4]暨南大学第一临床医学院,广东广州510632

出　　处：《分析测试学报》2012年第3期247-254,共8页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(30872404);"973计划"资助项目(2010CB/833603);中国博士后基金项目(20090450099;201003359)

摘　　要：利用原子力显微镜、CCK-8实验和流式细胞术研究了蝙蝠葛碱(dauricine)对B细胞淋巴瘤daudi细胞的细胞毒性。蝙蝠葛碱能显著抑制daudi细胞的增殖。CCK-8实验表明,细胞存活率与蝙蝠葛碱浓度存在时间依赖和剂量依赖关系。经10～50μmol/L的蝙蝠葛碱作用24 h后,daudi细胞存活率从(89.8±4.3)%降至(11.2±3.2)%;48 h后,存活率从(68.9±2.6)%降至(2.5±0.5)%。流式细胞术表明蝙蝠葛碱处理dau-di细胞24 h后,凋亡率从5.2%增至28.2%(60μmol/L)。AFM数据显示对照组细胞呈圆形,表面较光滑。经蝙蝠葛碱处理后,daudi细胞坍塌,超微结构显示细胞表面粗糙、凹凸不平。此外,经不同浓度蝙蝠葛碱作用的daudi细胞,其线粒体膜电位随着药物浓度的加大而降低。蝙蝠葛碱能显著抑制daudi细胞生长增殖。The toxicity of dauricine on daudi cell of B cell lymphoma was investigated by atomic force microscopy, CCK-8 assay and flow cytometer method. The results showed that daurieine could sup- press the proliferation of daudi cells. By CCK-8 assay, it was found that cell viability was related with the reaction time and drug concentration. When 10 -50 μmol/L dauricine was incubated with daudi cells for 24 h and 48 h, respectively, the cell viability declined from （ 89.8 ± 4.3 ） % to （11.2±3.2）% and （68.9±2.6）% to （2.5 ±0.5）% , respectively. In addition, daudi cells were cultured with different concentrations of dauricine for 24 h, the result revealed that the apoptosis rate increased from 5.2% to 28.2% （60 μmoL/L）. AFM data showed that control cells were round and the surface was smooth. After treated with daurieine, daudi cell collapsed, the ultrastructure re- vealed that cell surface was rough and full of bumps and holes. Furthermore, daudi cells were treated with different concentrations of dauricine, the mitochondrial membrane potential of cell decreased with the increase of the dauricine concentration. Dauricine significantly inhibited the growth and pro- liferation of daudi cells.

关 键 词：蝙蝠葛碱 原子力显微镜 细胞毒性 超微结构 DAUDI细胞 细胞凋亡 

分 类 号：O766.1[理学—晶体学] R361.3[医药卫生—病理学]