人眼巩膜组织中亮蛋白聚糖基因mRNA水平与高度近视及青光眼的相关性  

Detection of differentially expressed lumican gene mRNA level in scleral tissue in human eye

作　　者：赵燕燕[1] 张丰菊[1] 朱思泉[1] 唐昕[1] 孙丽[1] 王宁利[1] 室李宏[2] 刘佳[2] 孔庆友[2] 

机构地区：[1]首都医科大学附属北京同仁医院北京同仁眼科中心北京市眼科学与视觉科学重点实验室,100730 [2]大连医科大学基础医学院细胞生物学院教研

出　　处：《中华医学杂志》2012年第9期608-611,共4页National Medical Journal of China

基　　金：国家自然科学基金（30872834）

摘　　要：目的探讨人眼巩膜组织中亮蛋白聚糖基因mRNA水平与高度近视及青光眼的相关性。方法2009年10月至2010年12月在首都医科大学附属同仁医院住院行抗青光眼手术的原发性开角型青光眼患者21例及自愿捐献角膜的健康成年人12例（正视眼）。11例行抗青光眼手术的原发性开角型青光眼患者（不伴有高度近视）及10例行抗青光眼手术的原发性开角型青光眼患者（伴有高度近视）术中切除的眼前节巩膜组织。提取巩膜组织中总RNA，针对亮蛋白聚糖基因设计特异性引物进行逆转录多聚酶链反应（RT．PCR）检查，用凝胶分析系统测定各样本B肌动蛋白和亮蛋白聚糖基因PCR反应产物电泳条带灰度值，采用单因素方差分析进行比值（13肌动蛋白电泳条带灰度值／亮蛋白聚糖电泳条带灰度值）的统计分析。结果B肌动蛋白／亮蛋白聚糖比值在青光眼伴高度近视组[0．29±0．10]和青光眼组[0．29±0．10]之间比较，差异无统计学意义（P〉0．05），而与正常对照组[2．67±1．05]比较，差异有统计学意义（P〈0．01）。结论高度近视候选基因亮蛋白聚糖与高度近视可能不相关，但可能与青光眼存在一定相关性，尚有待进一步研究。Objective To identify differentially expressed lumcian in scleral tissue of eyes with primary open-angle glaucoma （POAG） and high mopia （HM）. Methods Total RNA was isolated from scleral tissue of cadaveric eyes derived from normal donors, patient＇s eyes with diagnosed glaucoma and high mopia who accepted trabeculectomy. RNA was amplified, RT-PCR was used to measure the levels of mRNA. The ratio of the electrophoresis strips＇gray scale values of the ^-actin over the lumican gene was obtained for ANOVA analysis. Results [3-actin/LUM of normal eye was significantly higher than that of POAG and POAG ＋ HM eyes （P 〈 0. 01 ）, but there was no significant difference between POAG and POAG ＋ HM eyes （P 〉 0. 05）. Conclusion Differentially expressed lumican between POAG and control groups identified in this study have not been previously investigated for their role in the pathogenesis of POAG and thus are novel factors for further study of the mechanism of the disease and for their possible use as diagnostic markers.

关 键 词：蛋白聚糖类 基因 近视 青光眼 开角型 逆转录聚合酶链反应 

分 类 号：R775[医药卫生—眼科]