组织间Duffy抗原／趋化因子受体在大鼠静脉高压炎症反应中的作用及其机制  

Experimental study of Duffy antigen receptor for chemokines in tissue and the inflammatory reaction of limb with venous hypertension

作　　者：秦委委[1] 张岚[1] 张纪蔚[1] 张柏根[1] 何以丰[2] 陈春妹[2] 余龙[2] 

机构地区：[1]上海交通大学医学院附属仁济医院血管外科,200127 [2]复旦大学生命科学学院遗传学研究所遗传工程国家重点实验室

出　　处：《中华医学杂志》2012年第9期638-641,共4页National Medical Journal of China

基　　金：上海市科委自然科学基金（10ZRl418800）

摘　　要：目的探讨组织间Duffy抗原／趋化因子受体（DARC）在下肢静脉高压炎症反应中的作用及其可能的机制。方法采用股动一静脉瘘建立大鼠后肢静脉高压模型，36只sD大鼠数字随机分为pcDNA3．1-DARC质粒组、pcDNA3．1空质粒组和对照组。术后14d和42d，采用实时定量聚合酶链反应（Real—PCR）和蛋白印迹（Westernblotting）检测组织间DARCmRNA和蛋白的表达；酶联免疫吸附测定法检测血清白细胞介素（IL）一8的含量；TUNEL法和HE染色检测组织凋亡和白细胞浸润情况。结果随着静脉高压时间的延长，组织间DARC表达量、组织凋亡及白细胞浸润程度在pcDNA3．1-DARC质粒组和pcDNA3．1空质粒组均呈增加的趋势，同期各组间比较，pcDNA3．1-DARC质粒组明显高于pcDNA3．1空质粒组和对照组，差异均有统计学意义（均P〈0．05）；血清IL-8含量在pcDNA3．1-DARC质粒组和pcDNA3．1空质粒组均表现为降低的趋势，同期各组间比较，pcDNA3．L．DARC质粒组明显低于pcDNA3．1空质粒组，但两组均高于对照组，差异均有统计学意义（均P〈0．05）。结论组织间DARC表达与静脉高压组织的炎症程度呈正相关，可能通过加强白细胞的黏附和迁移促进静脉高压炎症反应的发展。Objective To explore the role and mechanism of Duffy antigen receptor for chemokines （DARC） of tissue in promoting the inflammatory reaction of the limb with venous hypertension. Methods Femoral arteriovenous fistula was surgically created to establish the rat model of venous hypertension. A total of 36 SD rats were randomly divided into peDNA3.1-DARC （ Group A ）, empty plasmid of pcDNA3.1 （Group B） and control （Group C） groups. The animals were sacrificed at Days 14 and 42 post-operation respectively. The expressions of DARC at the RNA and protein level were detected by real-time polymerase chain reaction （Real-PCR） and Western blotting. And the serum level of interleukin （IL）-8 was detected by enzyme linked immunosorbent assay （ELISA） and the degrees of apoptosis and leukoeytic infiltration of local tissue were detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling （TUNEL） and hematoxylin and eosin （HE） staining, Results With the elapsing time of venous hypertension, the DARC expression in tissue, the extent of apoptosis and leukocytic infiltration in tissue showed an increasing trend in Groups A and B. Group A was obviously higher than Group B during the corresponding period. And the differences were statistically significant （ P 〈 0. 05 ）. The serum levels of IL- 8 of Groups A and B showed a decreasing trend. And Group A was obviously lower than Group B. Both groups were higher than the control group. The differences were statistically significant （P 〈 O. 05 ）. Conclusion The level of DARC in tissue and the degree of inflammatory reaction of venous hypertension bare a positive correlation. And DARC may promote the development of venous hypertension inflammation through augmenting the adhesion and migration of leukoeytes.

关 键 词：受体 趋化因子 静脉 炎症 白细胞介素8 Duffy抗原 

分 类 号：R363[医药卫生—病理学]