Effects of fulvestrant on biological activity and Wnt expression in rat GH3 cells  

作　　者：Jiwei Bai Yan Wang Chuzhong Li Yazhuo Zhang 

机构地区：[1]Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China [2]Center of Clinical Genetics, Affiliated Bayi Children＇s Hospital, General Hospital of Beijing Military Command of Chinese PLA, Beijing 100700, China

出　　处：《Neural Regeneration Research》2012年第4期283-289,共7页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China, No. 81072075

摘　　要：The present study investigated the influence of anti-estrogen treatment （fulvestrant） on pituitary adenoma cell line GH3 biological activity, the estrogen receptor a pathway, the WnT pathway, and mechanisms of decreased Wnt inhibitory factor-1 expression in GH3 cells. Results showed that fulvestrant suppressed GH3 cell proliferation and reduced hormone secretion in a dose-dependent manner. Estrogen receptor a and Wnt4 expression decreased, but Wnt inhibitory factor-1 expression increased in a dose-dependent manner following fulvestrant treatment, and β-catenin expression remained unchanged. Inhibitors of DNA methylation and histone modification upregulated Wnt inhibitory factor-1 expression. Results suggested that fulvestrant suppressed biological activity of GH3 cells via the estrogen receptor a and Wnt pathways. These results suggested that decreased Wnt inhibitory factor-1 expression in GH3 cells played a role in epigenetic mechanisms. Anti-estrogen therapies could provide novel treatments for growth hormone adenomas.The present study investigated the influence of anti-estrogen treatment （fulvestrant） on pituitary adenoma cell line GH3 biological activity, the estrogen receptor a pathway, the WnT pathway, and mechanisms of decreased Wnt inhibitory factor-1 expression in GH3 cells. Results showed that fulvestrant suppressed GH3 cell proliferation and reduced hormone secretion in a dose-dependent manner. Estrogen receptor a and Wnt4 expression decreased, but Wnt inhibitory factor-1 expression increased in a dose-dependent manner following fulvestrant treatment, and β-catenin expression remained unchanged. Inhibitors of DNA methylation and histone modification upregulated Wnt inhibitory factor-1 expression. Results suggested that fulvestrant suppressed biological activity of GH3 cells via the estrogen receptor a and Wnt pathways. These results suggested that decreased Wnt inhibitory factor-1 expression in GH3 cells played a role in epigenetic mechanisms. Anti-estrogen therapies could provide novel treatments for growth hormone adenomas.

关 键 词：β-catenin ESTROGEN estrogen receptor a GH3 cell line Wnt inhibitory factor-1 Wnt4 

分 类 号：Q2[生物学—细胞生物学] S482.39[农业科学—农药学]