FAP对体外内皮细胞增殖及其小管形成的影响  

Effects of FAP on human umbilical vein endothelial cells proliferation and angiogenesis in vitro

作　　者：朱琳[1] 张宁[2] 代海丽[1] 邵洪江[3] 赵艳[1] 靳占峰[1,3] 

机构地区：[1]哈尔滨医科大学病理学教研室,黑龙江哈尔滨150081 [2]哈尔滨医科大学病理生理学教研室,黑龙江哈尔滨150081 [3]哈尔滨医科大学法医学教研室,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2012年第1期10-13,18,共5页Journal of Harbin Medical University

摘　　要：目的探讨成纤维细胞激活蛋白(fibroblast activation protein,FAP)在体外对内皮细胞的增殖和小管形成的影响。方法应用不同浓度FAP(0、300、600、1 200 pmol/L)处理人脐静脉内皮细胞EA.hy926,采用MTT检测内皮细胞增殖,划痕愈合实验分析细胞迁移并使用三维胶模型研究内皮细胞的小管形成。结果与正常对照组相比,FAP可以诱导内皮细胞迁移和增殖(P<0.05),并促进内皮细胞小管形成(P<0.01),其中600 pmol/L浓度的FAP处理组作用较其他组明显增强(P<0.01)。结论 FAP在体外可促进人脐静脉内皮细胞增殖、迁移和小管形成。此发现为进一步研究FAP在血管生成中的机制提供了理论基础。Objective To investigate the effects of FAP on human umbilical vein endthelial cell proliferation and angiogenesis in vitro. Methods Human umbilical vein endothelial cell EA. hy926 was treated with different concentrations of FAP（0,300,600,1200 pmol/L）. Cell proliferation was determined by MTr assay. Cell migration was assessed by a wound healing as- say. And tube formation was studied with a three-dimensional gel model. Results Compared with the normal control group, FAP promoted endothelial cell migration and proliferation （P 〈 0. 05）, promoted tube formation of endothelial cell on the gel （P＇〈0. 01 ）. The effect of 600 pmol/L FAP was significantly higher than that of other groups （P 〈 0. 01 ）. Conclusion FAP can promote human umbilical vein endothelial cell proliferation, migration and angiogenesis in vitro. It provides a basic theoretical depiction of the role of FAP in angiogenesis.

关 键 词：内皮细胞 成纤维细胞激活蛋白 MTT 划痕实验 小管形成 

分 类 号：R361.3[医药卫生—病理学]