溶血磷脂酸受体1在人脑胶质瘤组织中的表达及意义  

Expression of lysophosphatidic acid receptor 1 in human brain glioma tissue and its significance

作　　者：杨志青[1] 刘辉[2] 许万振[1] 李蕴潜[1] 

机构地区：[1]吉林大学第一医院神经外科,长春130021 [2]吉林大学白求恩医学院解剖教研室,长春130021

出　　处：《中国生物制品学杂志》2012年第3期364-366,共3页Chinese Journal of Biologicals

基　　金：吉林省科技厅重点项目基金(20090452)

摘　　要：目的探讨溶血磷脂酸受体1(Lysophosphatidic acid receptor 1,LPAR1)在人脑胶质瘤组织中的表达及意义。方法收集临床切除的经病理证实的良性胶质瘤组织、恶性胶质瘤组织及正常脑组织,采用RT-PCR法及免疫组织化学染色法检测各组脑组织中LPAR1在mRNA及蛋白水平的表达,并分析LPAR1表达与脑胶质瘤临床病理特征之间的相关性。结果正常脑组织、良性胶质瘤组织、恶性胶质瘤组织中均有LPAR1 mRNA及蛋白的表达;胶质瘤组织中LPAR1 mRNA及蛋白的表达水平均明显高于正常脑组织,差异有统计学意义(P<0.05),且恶性胶质瘤组织中LPAR1蛋白的表达水平明显高于良性胶质瘤组织(P<0.05);LPAR1的表达与脑胶质瘤临床病理分级相关,而与年龄、性别、肿瘤部位无关。结论 LPAR1在脑胶质瘤组织中表达增高,可能在胶质瘤发生发展的过程中发挥重要作用。Objective To investigate the expression of lysophosphatidic acid receptor 1（LPAR1） in human brain glioma tissue as well as its significance.Methods Normal human brain tissue as well as benign and malignant glioma tissues confirmed by pathological examination were collected from surgery,and determined for expressions of LPAR1 at mRNA and protein levels by RT-PCR and immunohistochemical staining respectively,then analyzed for relationship between LPAR1 expression and clinical pathological character of brain glioma.Results LPAR1 was expressed at both mRNA and protein levels in all the normal brain and glioma tissues.However,both the expression levels of LPAR1 mRNA and protein were significantly higher in glioma tissue than in normal brain tissue（P 0.05）,and the expression level of LPAR1 protein was significantly higher in malignant than in benign glioma tissue（P 0.05）.The expression of LPAR1 was related to the clinical pathological stage of brain glioma,while showed no relationship to the age,sex or site of glioma of patients.Conclusion The expression level of LPAR1 increased in brain glioma tissue,indicating possible important role of LPAR1 in onset and progress of glioma.

关 键 词：胶质瘤 受体 溶血磷脂酸 逆转录聚合酶链反应 免疫组化 

分 类 号：R739.41[医药卫生—肿瘤]