Beclin1和PTEN蛋白在胃癌中的表达及预后价值  

The expression and prognostic value of Beclin 1 and PTEN in gastric carcinoma

作　　者：李捷[1] 于观贞[1] 王杰军[1] 潘军[1] 柳珂[1] 李萍[1] 周洲[1] 陈颖[2] 

机构地区：[1]上海长征医院肿瘤科,200070 [2]上海长海医院病理科

出　　处：《中华消化杂志》2012年第3期170-174,共5页Chinese Journal of Digestion

摘　　要：目的探讨Beclin1和PTEN蛋白在胃癌发生过程中的意义及其对预后的影响。方法应用组织芯片和免疫组织化学法检测199例胃癌及相应癌旁正常组织中Beclin1和PTEN蛋白的表达，分析其与胃癌的关系；用Western印迹方法检测15例新鲜胃癌及相应癌旁组织中Beclin1和PTEN蛋白的表达率。所有标本均来自上海长征医院。结果免疫组织化学法检测提示癌组织中Beclin1和PTEN蛋白阳性表达率分别为47．2％（94／199）和55．8％（111／199），均低于相应癌旁组织的表达率E94．5％（188／199）和92．5％（184／199），P〈0．01]。胃癌组织中Beclin1和PTEN蛋白的低表达均与性别、分化程度、肿瘤浸润深度、淋巴结转移以及疾病分期有关（P〈0．05）。胃癌组织中Beclin1和PTEN的表达呈正相关（r=0．680，P〈0．01）。生存分析表明Beclin1和PTEN均是判断胃癌患者预后的独立因素。Beclin1阳性患者的5年生存率为67．0％（63／94），阴性者为33．30（35／105）；PTEN阳性患者的5年生存率为71．2％（79／111），阴性者为21．60（19／88）（P值均d0．01）。Western印迹方法检测结果提示胃癌标本中Beclin1和PTEN蛋白含量明显低于相应癌旁正常组织（P值均G0．01）。结论Beclin1和PTEN的异常表达可能和胃癌的发生发展相关。Objective To explore the role of Beclin 1 and PTEN in gastric caicinoma genesis and the effects on prognosis. Methods The expression of Beclin 1 and PTEN in 199 gastric caicinoma specimens and corresponding adjacent noncancerous tissues were examined by tissue microarrays and immunohistochemistary, and the relation with gastric cancer was analyzed. The rate of Beclin 1 and PTEN expression in 15 fresh gastric carcinoma samples and corresponding adjacent noncancerous tissues were detected by Western blot. All the samples were from Changzheng Hospital. Results The Results of immunohistochemistary showed that the rates of Beclin 1 and PTEN positive expression in cancinoma tissues were 47. 2% （94/199） and 55. 8% （111/199）, both were lower than that of adjacent noncancinoma tissues （94. 5%, 188/199 and 92. 5%, 184/199; P~ 0. 01）. The lower expression of Beclin 1 and PTEN in gastric carcinoma were associated with gender, differentiation degree, depth of tumor invasion, lymph node metastases and disease stage（P〈0.05）. There was a positive correlation between Beclin 1 and PTEN expression in gastric carcinoma tissues （r =0. 680, P〈0. 01）. The survival analysis indicated that Beclin 1 and PTEN were independent factors in determining the prognosis of gastric cancinoma patients. The 5-year survival rate of Beclin 1 positive patients was 67.0% （63/94）, and of negative patients was 33.3% （35/105）. The 5-year survival rateof PTEN positive patients was 71.2% （79/111）, and of negative patients was 21.6%（19/88） （ all P〈0. 001）. The Results of Western blot indicated that the expression of Beclin 1 and PTEN in gastric carcinoma tissues were significantly lower than that in the adiacent nonearcinoma tissues （ all P〈0. 001）. Conclusion The abnormal expression of Beclin 1 and PTEN may be related to carcinogenesis and the development of gastric carcinoma.

关 键 词：胃肿瘤 自噬 肿瘤抑制蛋白质类 免疫组织化学 预后 

分 类 号：R735.2[医药卫生—肿瘤]