乳腺癌患者白细胞来源微泡的特性及其临床意义  

The characteristics of microparticles derived from leukocytes of patients with breast cancer and their clinical significance

作　　者：王宁方[1] 李慧玉[1] 杜雯[1] 李小青[1] 郑金娥[1] 张佳华[1] 黄士昂[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院干细胞研究与应用中心,武汉430022

出　　处：《肿瘤》2012年第3期208-213,共6页Tumor

基　　金：国家自然科学基金资助项目(编号:81072170)

摘　　要：目的:探讨乳腺癌患者白细胞来源微泡(leukocyte-derived microparticles,LMPs)的特性及其临床意义。方法:用流式细胞仪检测44例乳腺癌患者和10例健康志愿者外周血的LMPs以及其中CD44+LMPs、CD24+LMPs和CD44+CD24-LMPs的表达情况。进一步用流式细胞仪检测乳腺癌患者和志愿者外周血单个核细胞(monocytes,MNCs)中CD44+、CD24+和CD44+CD24-细胞的比例。最后分析LMPs的临床相关性以及MNCs与LMPs之间的相关性。结果:乳腺癌组的LMPs、CD44+LMPs和CD44+CD24-LMPs比例显著高于健康对照组(P值分别为0.021、0.011和0.006)。LMPs比例在不同大小的肿瘤组织间差异有统计学意义(P=0.018)。CD44+LMPs比例在不同水平的Her-2表达组间差异也有统计学意义(P=0.020)。MNCs中CD44+、CD24+和CD44+CD24-细胞比例在健康对照组和乳腺癌组之间差异无统计学意义。LMPs与MNCs间无显著相关性。结论:乳腺癌患者的LMPs比例与肿瘤大小有关,而CD44+LMPs比例与Her-2水平密切相关,该指标可用来评估乳腺癌的复发转移及预后情况。Objective：To explore the characteristics of leukocyte-derived microparticles（LMPs） in breast cancer patients and their clinical significance.Methods：The expressions of LMPs,CD44＋ LMPs,CD24＋ LMPs and CD44＋ CD24-LMPs in peripheral blood of 44 breast cancer patients and 10 healthy volunteers were detected by using flow cytometry.The percentages of CD44＋,CD24＋ and CD44＋ CD24-cells in monocytes（MNCs） of breast cancer pateints and healthy volunteers were further detected.The clinical relevance of LMPs,and the correlation between MNCs and LMPs were analyzed.Results：The percentages of LMPs,CD44＋ LMPs and CD44＋ CD24-LMPs in breast cancer patients were statistically higher than those in the healthy volunteers（P=0.021,P=0.011,and P=0.006,respectively）.There was a statistical difference in the percentage of LMPs among the different tumor volunmes（P=0.018）.Statistical difference was also obseved in the percentage of CD44＋ LMPs among different Her-2 levels（P=0.020）.The percentages of CD44＋,CD24＋ and CD44＋ CD24-cells in MNCs in breast cancer patients were not statistically different from those in the healthy volunteers.There was no statistical correlation between MNCs and LMPs.Conclusion：The percentage of LMPs in breast cancer patients can be used to evaluate the tumor volume.The percentage of CD44＋ LMPs was closely correlated with Her-2 level,and it can be used to evaluate the recurrence,metastasis and prognosis of breast cancer.

关 键 词：乳腺肿瘤 抗原 CD44 抗原 CD24 单核细胞 流式细胞术 白细胞来源的微泡 

分 类 号：R737.9[医药卫生—肿瘤]