老年人群klotho G-395A、F352V与C370S基因多态性及组合与其血清klotho蛋白水平的相关性研究  

Association of single nucleotide polymorphisms and combined genotypes of klotho G-395A,F352V and C370S with serum klotho protein levels in elderly people of Chinese Han population in Chongqing

作　　者：周平[1] 田小春[1] 马厚勋[1] 刘晓林[1] 翟福利[1] 吴平[1] 李法琦[1] 

机构地区：[1]重庆医科大学附属第一医院老年病科,重庆400016

出　　处：《重庆医科大学学报》2012年第1期21-24,共4页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(编号:30672212)

摘　　要：目的:探讨重庆市汉族老年人群Klotho G-395A、F352V与C370S 3个位点的单核苷酸多态性(Single nucleotide polymorphism,SNP)及其组合与血清klotho蛋白水平的相关性。方法:随机选择汉族老年人390例,采用等位基因特异性引物PCR技术检测Klotho G-395A、F352V与C370S SNP,酶联免疫吸附试验(Enzyme-linked immunosorbent assay,ELISA)检测其血清klotho蛋白水平。结果:Klotho G-395A、F352V与C370S SNP各等位基因型携带者其血清klotho蛋白水平均无显著性差异(P>0.05)。基因组合分析研究发现Klotho G-395A、F352V与C370S SNP各组合等位基因型携带者其血清klotho蛋白水平差异性较大,其中Klotho G-395G+F352F+C370S携带者较Klotho G-395G+F352F+C370C携带者其血清klotho蛋白水平明显增高(P=0.056)。结论:Klotho G-395A、F352V与C370S SNP各等位基因型携带者其血清klotho蛋白水平无显著性差异,而组合等位基因型研究发现Klotho G-395G+F352F+C370S携带者较Klotho G-395G+F352F+C370C组合等位基因型携带者血清klotho蛋白水平增高有显著性趋势。Objective：To investigate the association between serum klotho protein level and single nucleotide polymorphisms（SNPs） and combined genotypes of Klotho G-395A,F352V and C370S in elderly people of Han nationality in Chongqing.Methods：Unrelated 390 elderly individuals from Chinese Han population（in Chongqing were selected.Single nucleotide polymorphisms of Klotho gene in the G-395A,F352V and C370S were tested by allele-specific primer（ASP） PCR,and serum klotho protein level was determined by enzyme-linked immunosorbent assay（ELISA）.Results：No significant difference was found in serum klotho protein level in the aged carrying allelic genotypes of Klotho G-395A,F352V and C370S SNP（P0.05）.According to combined genotype analysis,the klotho protein level in the elderly individuals carrying Klotho G-395G＋F352F ＋C370S was higher than that in the elderly individuals with Klotho G-395G＋F352F＋C370C（P=0.056）.Conclusion：No association of allelic genotypes of Klotho G-395A,F352V and C370S SNP with serum klotho protein levels is found.But a trend is found that serum klotho protein level in those with Klotho G-395G＋F352F＋C370S is higher than that in the elderly individuals with Klotho G-395G＋F352F＋C370C.

关 键 词：KLOTHO基因 单核苷酸多态性 组合基因型 老年人 

分 类 号：R394.2[医药卫生—医学遗传学]