登革病毒中国株E基因重组的生物信息学分析  

Bioinformatics analysis of envelope gene recombination of Chinese dengue virus isolates

作　　者：涂增[1] 刘利成[2] 白志军 方美玉 陈唯军[2] 

机构地区：[1]重庆医科大学基础医学院病原生物学教研室,重庆400016 [2]中国科学院北京基因组研究所,北京101300 [3]广州医学研究所,广州510507

出　　处：《重庆医科大学学报》2012年第1期67-70,共4页Journal of Chongqing Medical University

基　　金：重庆医科大学院青年教师科研基金资助项目(编号:0800280002)

摘　　要：目的:了解我国登革病毒(Dengue virus,DV)包膜蛋白基因(Envelope,E)重组的发生情况。方法:从GenBank收集共70个DV中国株的E基因序列,Clustal-W比对后,重组检测软件包RDP3检测E基因中可能存在的重组事件,并经Mega4构建系统发生树验证所检测的重组事件的可靠性和真实性。结果:DV-1型中E基因区存在3个重组事件,共4个重组子,分别是1995、1997、1999年和2004年分离的DV病毒株,在其它3个血清型的DV病毒株中没有检测到重组,也未检测到血清型间重组。结论:我国DV 1型血清型E基因区域存在基因重组。Objective：To explore the recombination of envelope protein（E） gene of Chinese dengue virus（DV） isolates.Methods：70 Chinese DV E gene sequences available from GenBank were collected,and aligned with Clustal-W,then were incorporated into recombination detection program（RDP3） for potential recombination event detection.Phylogenetic trees constructed by Mega4 were further generated to confirm the recombination events.Results：Three intra-serotype recombination events,four recombinants isolated in 1995,1997,1999,and 2004,respectively,were confirmed in DV-1 E gene data.However,no recombination event was detected in other three serotypes in the data,so was the inter-serotype recombination.Conclusion：Recombination of E gene regions of Chinese dengue virus type 1 exists,although limited data were available.It highlights the necessity of employing more gene regions and sequences in the analysis to fully detect the recombination in China.

关 键 词：RDP3 登革热 E基因 遗传重组 

分 类 号：R373.3[医药卫生—病原生物学]