Hsp70对低氧大鼠海马DG区神经细胞p53、p-p38表达的研究  

The Overexpression of HSP70 of the Neurocyte in the Area of Dentate Gyrus(DG)on the p53and p-p38 of Rat's Hippocampus Under Hypoxia

作　　者：徐强 董大海 高丽莉[2] 徐文[2] 高琨[2] 罗芳[2] 侯顺利[2] 左晶[2] 刘红[2] 闫文[2] 杨银书[2] 卢娟[2] 

机构地区：[1]兰州市中医医院,甘肃兰州730000 [2]兰州军区疾病预防控制中心,甘肃兰州730020

出　　处：《现代生物医学进展》2012年第3期419-421,共3页Progress in Modern Biomedicine

基　　金：全军重点基金课题(G-867)

摘　　要：目的:探讨热休克蛋白70(HSP70)高表达对低氧大鼠海马DG区神经细胞p53、p-p38表达的影响。方法:将大鼠海马DG区神经细胞在41℃温浴1h诱导HSP70高表达,然后低氧培养,Western-blot检测细胞HSP70、p53、p-p38的表达。结果:温浴可以诱导HSP70高表达;HSP70高表达可抑制p53和p-p38表达。结论:HSP70保护低氧诱导的细胞凋亡可能与抑制p53和p-p38表达有关。Objective： To investigate the effect of the HSP70 over-expression on the p53and p-p38 of the neurocyte in the area of dentate gyrus（DG）of rat＇s hippocampus under hypoxia.Methods： Neurocyte in the area of dentate gyrus（DG）of rat＇s hippocampus was treated in 41℃ for 1h to induced the over-expression of HSP70.Culturing in hypoxiathe.The level of p53 and p-p38 was detected by Western-blot.Results： Warm bath could induced the over-expression of HSP70;The overexpression of HSP70 down-regulated p53 and p-p38.Conclusions： The over-expression of HSP70 inhibited cell apoptosis from hypoxia maybe related to the downregulating of p53 and of p-p38.

关 键 词：热休克蛋白70 细胞凋亡 P53 P-P38 

分 类 号：Q953[生物学—动物学] R733[医药卫生—肿瘤]