鼻咽癌患者唾液和血清及外周血白细胞中EBV-DNA的定量检测  

Quantitative analysis of Epstein-Barr virus DNA in saliva,blood serum and peripheral blood cells in patients with nasopharyngeal carcinoma

作　　者：张丽卫[1] 罗碧强[2] 窦晓清[2] 邓意红[2] 曾志梅[2] 李建忠[2] 明小兵[3] 陈贤明[1,2] 

机构地区：[1]福建医科大学福总临床医学院耳鼻咽喉头颈外科,福建福州350025 [2]南京军区福州总医院耳鼻咽喉头颈外科,福建福州350025 [3]南京军区福州总医院医学分子实验中心,福建福州350025

出　　处：《中国耳鼻咽喉颅底外科杂志》2012年第1期24-27,共4页Chinese Journal of Otorhinolaryngology-skull Base Surgery

摘　　要：目的对鼻咽癌患者唾液、血清及外周血白细胞中的EBV-DNA进行检测,分析唾液、血清、外周血白细胞中EB病毒的DNA含量与鼻咽癌的相关性。方法对40例初诊鼻咽癌患者和50例正常人的唾液、血清及外周血白细胞中的EBV-DNA进行荧光聚合酶链反应(FQ-PCR)检测。结果EBV-DNA在鼻咽癌患者血清中的检出率为67.5%,显著高于正常人血清中的EBV-DNA检出率2%(χ2=44.48,P<0.001);鼻咽癌患者的唾液、外周血白细胞EBV-DNA检出率与正常人相比,无显著性差异;鼻咽癌患者和正常人的唾液EBV-DNA拷贝数与外周血白细胞EBV-DNA拷贝数呈正相关性;鼻咽癌患者血清EBV-DNA拷贝数与TNM分级有关,分级越晚含量越高。结论 EB病毒长期存在于多数人的唾液中,也可存在于外周血B淋巴细胞中。血清EBV-DNA检测在鼻咽癌的临床诊断中具有较高的敏感性和特异性,有助于鼻咽癌的早期诊断;血清EBV-DNA拷贝数与TNM分级有关,可在分子水平对鼻咽癌的TNM分级进行补充。To quantitatively analyze DNA levels of Epstein Barr virus （EBV） in saliva, blood serum, and peripheral blood cells （ PBCs ） of nasopharyngeal carcinoma （ NPC ） patients, and to investigate the relationship between EBVDNA levels and NPC. Methods EBVDNA levels in samples of saliva, blood serum and PBCs from 40 primary NPC patients and 50 controls were quantitatively detected with real-time quantitative PCR （FQ-PCR） respectively. Results The detective rate of EBVDNA in blood serum from NPCs （67. 5 % ） was significantly higher than that in the controls 2% （ X2 = 44. 48, P 〈 0. 001 ）. The differences of EBVDNA detective rates in the saliva, PBCs between the NPCs and control group were statistically insignificant. Saliva EBVDNA level was correlated with PBCs EBV-DNA load in both NPCs and the control. In NPC patients, blood serum EBVDNA level was significantly increasing with the advance in TNM stage. Conclusion Epstein Barr virus may exist in saliva and peripheral blood B lymphocyte in most normal people. With high sensitivity and specificity, the blood serum EBV-DNA examination may be helpful in the early diagnosis of NPC, and also facilitate the TNM stagingof NPC in clinical practice.

关 键 词：鼻咽癌 EB病毒脱氧核糖核酸 血清 唾液 外周血白细胞 

分 类 号：R739.63[医药卫生—肿瘤]