BMP-2和p21^(WAF1)的表达与乳腺癌临床病理特征及其发生骨转移的关系  

EXPRESSIONS OF BMP -2 AND p21^(WAF1) IN BREAST CANCER WITH BONE METASTASIS

作　　者：荀建军[1] 李艳芳[2] 贾志峰[1] 冯建刚[1] 

机构地区：[1]河北医科大学第四医院骨科,河北石家庄050011 [2]河北医科大学第二医院妇科,河北石家庄050000

出　　处：《河北医科大学学报》2012年第1期31-35,共5页Journal of Hebei Medical University

基　　金：河北省2009年医学科学研究重点课题计划项目(20090160)

摘　　要：目的检测骨形成蛋白-2(bone morphogenetic protein-2,BMP-2)和p21^(WAF1)在乳腺癌患者原发灶及骨转移灶中的表达,探讨它们在乳腺癌骨转移发生中的意义。方法选择临床及病理资料完整的乳腺癌患者126例,其中未发生骨转移者88例,发生骨转移者38例。应用免疫组织化学方法检测原发灶及骨转移灶中BMP-2和p21^(WAF1)的表达情况,并结合患者年龄、肿瘤大小、淋巴结转移、临床分期等,探讨它们之间的相互关系。结果①乳腺癌组织中BMP-2和p21^(WAF1)的阳性表达率在有骨转移、有淋巴结转移组中均低于无骨转移、无淋巴结转移组(P<0.01)。②临床Ⅲ～Ⅳ期的乳腺癌组织BMP-2和p21^(WAF1)的阳性表达率分别为20.59%、23.53%,明显低于其在临床Ⅱ期乳腺癌组织中的表达(P<0.01)。③BMP-2阳性表达率在骨转移灶中的表达明显低于其在原发灶中的表达(P<0.05),p21^(WAF1)阳性表达率在骨转移灶中也低于原发灶,但差异无统计学意义(P>0.05)。④BMP-2和p21^(WAF1)蛋白阳性表达率呈正相关(r=0.887,P<0.01)。结论①乳腺癌合并淋巴结转移时骨转移的发生率高。②临床分期越晚,骨转移的发生率越高。③BMP-2和p21^(WAF1)高表达着淋巴结转移少,发生骨转移的机会较少;而BMP-2和p21^(WAF1)的低表达预示具有形成骨转移的潜能。Objective To investigate the expression and significance of 1021wan and bone morphogenetic protein -2 （BMP -2） with bone metastasis in patients with breast cancer. Methods A total of 126 breast cancer patients including 38 cases with bone metastasis were analysed. The expressions of BMP -2 and p21wAF1 were detected by immunohistochemical staining. The correlations among the expressions of BMP - 2, p21WAF1 and clinical stage, positive lymph node and tumor size were analysed. Results （1）The positive expression of BMP - 2 and p21wan in the group of breast cancer with bone metastasis was lower than that in the group without bone metastasis （ P 〈 0.01 ）. The same result was obtained between the group of breast cancer with axillary lymph node metastasis and the group of no lymph node metastasis. （2）The positive expression of BMP - 2 and p21 WAF1 in stages Ⅲ - Were 20.59% and 23.53% respectively and siginificantly lower than those of stage Ⅱ （ P 〈 0.01 ）. （3）The positive expression of BMP-2 in tissue of bone metastasis was lower than that of breast cancer（P 〈 0.05 ）. Thepositive expression of p2WAF1 in tissue of bone metastasis was lower, too, but there was no significant difference （ P 〉 0. 05 ）. 4There was a positive correlation between the expression of BMP - 2 and p21 WAF1 （ r = 0. 887,P 〈 0. 01 ）. Conclusion Patients with high expression of BMP -2 and p21 WAF1 have less risk of lymph node metastasis and bone metastasis. The low expression of BMP - 2 and p21 WAF1 may indicate the potential of bone metastasis.

关 键 词：骨形态发生蛋白质类 乳腺肿瘤 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]