Exogenous plant MIR168a specifically targets mammalian LDLRAPI： evidence of cross-kingdom regulation by microRNA  

作　　者：Lin Zhang Dongxia Hou Xi Chen Donghai Li Lingyun Zhu Yuj ing Zhang Jing Li Zhen Bian Xiangying Liang Xing Cai Yuan Yin Cheng Wang Tianfu Zhang Dihan Zhu Dianmu Zhang Jie Xu Qun Chen Yi Ba Jing Liu Qiang Wang Jianqun Chen Jin Wang Meng Wang Qipeng Zhang Junfeng Zhang Ke Zen Chen-Yu Zhang 

机构地区：[1]Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Bio- technology, School of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing, Jiangsu 210093, China [2]Department of Chem- istry and Biology, School of Science, National University of Defense Technology, Changsha, Hunan 410073, China [3]Tianjin Medical University Cancer Institute and Hospital, Huanhuxi Road, Tiyuanbei, Tianjin 300060, China

出　　处：《Cell Research》2012年第1期107-126,共20页细胞研究（英文版）

基　　金：We thank Dr Daniel Wu （Yale University, USA） and Dr Zheng- Gang Liu （Cancer Research Institute, NIH, USA） for critical reading of the manuscript and discussion of data. This work was supported by grants from the National Natural Science Foundation of China （90813035, 30890044, 30771036, 30772484, 30725008, 30890032, 31071232, 31000323, and 90608010）, the National Basic Research Program of China （973 Program 2006CB503909, 2007CB815701, 2007CB815703, 2007CB815705, and 2007CB815804; 863 Program 2006AA02Z177 and 2006AA10A121）, and the Natural Science Foundation of Jiangsu Province （BK2008021）.

摘　　要：Our previous studies have demonstrated that stable microRNAs （miRNAs） in mammalian serum and plasma are actively secreted from tissues and cells and can serve as a novel class of biomarkers for diseases, and act as signaling molecules in intercellular communication. Here, we report the surprising finding that exogenous plant miRNAs are present in the sera and tissues of various animals and that these exogenous plant miRNAs are primarily acquired orally, through food intake. MIR168a is abundant in rice and is one of the most highly enriched exogenous plant miRNAs in the sera of Chinese subjects. Functional studies in vitro and in vivo demonstrated that MIR168a could bind to the human/mouse low-density lipoprotein receptor adapter protein 1 （LDLRAP1） mRNA, inhibit LDLRAP1 expression in liver, and consequently decrease LDL removal from mouse plasma. These findings demonstrate that ex- ogenous plant miRNAs in food can regulate the expression of target genes in mammals.

关 键 词：MICRORNA MIR168a LDLRAP1 low-density lipoprotein MICROVESICLE cross-kingdom 

分 类 号：Q522[生物学—生物化学] S451[农业科学—植物保护]