Direct reprogramming of Sertoli cells into multipotent neural stem cells by defined factors  

作　　者：Chao Sheng Qinyuan Zheng Jianyu Wu Zhen Xu Libin Wang Wei Li Haijiang Zhang Xiao-Yang Zhao Lei Liu Ziwei Wang Changlong Guo Hua-Jun Wu Zhonghua Liu Liu Wang Shigang He Xiu-Jie Wang Zhiguo Chen Qi Zhou 

机构地区：[1]State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China [2]College of Life Science, Northeast Agricultural University of China, Harbin 150030, China [3]Center for Regenerative Medicine, Beijing Institute of Geriatries, Xuanwu Hospital, Capital Medical University, Beijing, China, and Key Laboratory of Neurodegen- eration, Ministry of Education, Beijing 100053, China [4]State Key Laboratory of Brain and Cognitive Sciences, Institute of Bio- physics, Chinese Academy of Sciences, Beijing 100101, China [5]State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China [6]Graduate School of Chinese Academy of Sciences, Beijing 100049, China

出　　处：《Cell Research》2012年第1期208-218,共11页细胞研究（英文版）

基　　金：This study was supported in part by grants from the National Science Foundation of China 90919060 （to Q Z） and 31070946 （to Z C）, the ＂Strategic Priority Research Program＂ of the ChineseAcademy of Sciences XDA01020100 （to Q Z）, and the China National Basic Research Program 2007CB947702 （to L W）, 2011CBA01101 （to Q z）, and 2011CB965103 （to Z C）. We thank Yu Ren, Xiaona Wang, Yixun Liu, Wenhui Nai and Y Alex Zhang for their helpful discussion. There is no conflict of interest declared by any of the authors.

摘　　要：Multipotent neural stem/progenitor cells hold great promise for cell therapy. The reprogramming of fibroblasts to induced pluripotent stem cells as well as mature neurons suggests a possibility to convert a terminally differenti- ated somatic cell into a multipotent state without first establishing pluripotency. Here, we demonstrate that sertoli cells derived from mesoderm can be directly converted into a multipotent state that possesses neural stem/progenitor cell properties. The induced neural stem/progenitor cells （iNSCs） express multiple NSC-specific markers, exhibit a global gene-expression profile similar to normal NSCs, and are capable of self-renewal and differentiating into glia and electrophysiologically functional neurons, iNSC-derived neurons stain positive for tyrosine hydroxylase （TH）, y-aminobutyric acid, and choline acetyltransferase. In addition, iNSCs can survive and generate synapses following transplantation into the dentate gyrus. Generation of iNSCs may have important implications for disease modeling and regenerative medicine.

关 键 词：direct conversion neural stem cell MULTIPOTENT TRANSDIFFERENTIATION TRANSPLANTATION 

分 类 号：Q78[生物学—分子生物学] Q421