高静压处理对芸豆分离蛋白的聚集行为研究  

Effect of High Pressure on Aggregation Behavior of Kidney Bean(Phaseolus Vulgaris L) Protein Isolate

作　　者：黎亢抗[1] 马雯[1] 尹寿伟[1] 唐传核[1] 杨晓泉[1] 齐军茹[1] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640

出　　处：《现代食品科技》2012年第3期252-255,共4页Modern Food Science and Technology

基　　金：广东省自然科学基金资助项目(10451064101005149)

摘　　要：采用排阻色谱-多角度激光光散射-折光联用技术(SEC-MALLS-RI)研究高静压处理(HP)对芸豆分离蛋白分子量分布的影响规律,揭示HP导致KPI聚集-解离行为。根据Debye plot方法,计算出芸豆球蛋白的绝对分子量为161kDa,200MPa HP处理解离KPI的可溶性聚集物,而400和600MPa HP处理诱导不溶性聚集物向可溶性聚集物转化,改善KPI的溶解度(PS)。The influence of high pressure （HP） treatment （200-600 MPa） on molecular distribution of kidney bean （Phaseolus vulgaris L） protein isolate （KPI） was investigated by High-performance size-exclusion chromatography combined with multi-angle laser light seattering technique （HPSEC-MALLS）, to explore the possible HP-induced protein association-dissociation behavior. The molecular weight （Mw） of phaseolin, the major component in KPI, calculated based on the Debye plot, was about 161 kDa. HP treatment at 200 MPa led to dissociation of soluble aggregates （at void volume）, while HP treatment at 400 and 600 MPa led to dissociation of insoluble aggregates in KPI, thus improved PS.

关 键 词：芸豆分离蛋白 聚集 解离 高静压 溶解度 

分 类 号：S643.9[农业科学—蔬菜学]