TopoⅡα在宫颈癌前病变中的表达及其与高危型人乳头瘤病毒感染的关系  

Expression of Topo Ⅱα in Cervical Intraepithelial Neoplasia and Its Relation to High-risk Human Papillomavirus Infection

作　　者：屈王蕾[1] 高宝辉[2] 董剑达[3] 吕杰强[1] 

机构地区：[1]温州医学院附属第二医院妇产科,325027 [2]温州医学院附属第二医院病理科,325027 [3]温州医学院附属第二医院肿瘤外科,325027

出　　处：《医学研究杂志》2012年第2期25-28,共4页Journal of Medical Research

基　　金：英国威康信托基金会资助项目(071845/Z/03/Z);浙江省计划生育委员会资助项目(200930)

摘　　要：目的探讨TopoⅡα蛋白在宫颈癌前病变组织中的表达及其在宫颈癌发生中的作用,并探讨高危型人乳头瘤病毒感染与TopoⅡα表达的关系。方法选取2006年1～12月在温州医学院附属第二医院行宫颈病变诊治的患者,其中包括各级宫颈上皮内瘤变(CIN)和宫颈鳞癌(SCC)的患者。随机选取同期就诊的慢性宫颈炎患者作为对照。运用第2代杂交捕获法(HC2)检测高危型人乳头瘤病毒(HR-HPV)的表达。活体提取组织,运用免疫组织化学法(SP法)检测宫颈组织中TopoⅡα蛋白表达。结果 TopoⅡα在正常宫颈上皮组织、CINⅠ、CINⅡ、CINⅢ、SCC中的表达率分别20.0%(6/30)、15.8%(6/38)、46.9%(15/32)、85.7%(30/35)和90.0%(27/30),表达率逐渐增加,有统计学意义。在HR-HPV阳性患者的宫颈病灶组织中TopoⅡα蛋白阳性表达率高于HR-HPV阴性患者,有统计学意义。结论 TopoⅡα蛋白表达随着宫颈癌前病变的严重程度而上调,可能参与宫颈上皮的异常增殖和恶性转变。其表达的上调与HR-HPV感染有关,可能是HR-HPV感染后导致细胞恶性转化的中间行为。Objective To investigate the expression of topoisomerase Ⅱα in cervical intraepithelial neoplasia（CIN） and invasive squamous cell carcinoma（SCC） and the function of cervical carcinogenesis;and study the influence of high-risk human papillomavirus（HR-HPV） infection on it.Methods Totally 135 patients with CIN or SCC were rolled in the Second Affiliated Hospital of Wenzhou Medical College from January to December in 2006.Another 30 patients during the same period with chronic cervictits were selected as control group.Hybird capture Ⅱ assay（HC2）was used to detect the expression of HR-HPV of all the patients.All tissues were diagnosed and grouped through HE after operation.Immunohistochemical staining（SP） was used to detect the topoisomerase Ⅱα in these tissues.Results The positive rate of expression of Topo Ⅱα in NCE,CIN Ⅰ,CINⅡ,CINⅢ and SCC was 20.0%（6/30）,15.8%（6/38）,46.9%（15/32）,85.7%（30/35） and 90.0%（27/30）,respectively.A progressive increase was along with the grade of the lesion（P0.05）.The positive rate of expression of TopoⅡα was increased in HR-HPV-positive lesions than that of HR-HPV-negative（64.2% vs 13.3%,P0.05）.The increased TopoⅡα expression was associated with HR-HPV（rs=0.454,P0.05）.Conclusion Topo Ⅱα expression was up-regulated with the increasing grade of cervical lesion.TopoⅡα could be involved in abnormal proliferation and cancerization of cervix epithelium.The up-regulation of TopoⅡα was related to HR-HPV infection and could be a stage of cancerization after HR-HPV infection.

关 键 词：TopoⅡα 宫颈癌 人乳头瘤病毒 

分 类 号：R737.33[医药卫生—肿瘤]