不同相对分子质量岩藻聚糖硫酸酯抗血栓活性比较及机制探讨  

A Comparative Study of Antithrombotic Activities of Fucoidan with Different Molecular Weight and Its Mechanism

作　　者：陈安进[2,3] 赵雪[1,3] 张芳[3] 

机构地区：[1]中国海洋大学,食品科学与技术学院,山东肯岛266061 [2]中国海洋大学,医药学报院,山东肯岛266061 [3]青岛市市立医院药学部,山东青岛266071

出　　处：《中国药学杂志》2012年第6期431-434,共4页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(30800858);山东省自然科学基金(ZR2010CQ020)

摘　　要：目的研究不同相对分子质量岩藻聚糖硫酸酯抗血栓作用并从抗血小板聚集和纤溶两个方面探讨其作用机制。方法采用FeCl3诱导大鼠颈动脉血栓模型,考察4种相对分子质量岩藻聚糖硫酸酯的抗血栓形成作用;并通过采用酶联免疫吸附测试法(ELISA)测定动物血浆血栓素B2(TXB2)、6-酮-前列腺素F1α(6-keto-PGF1)和纤维蛋白溶酶原(PLG)、组织型纤维蛋白溶酶原活化剂(t-PA)、纤溶酶原激活物抑制剂1(PAI-1)几个指标观察各组药物对抗血小板聚集及纤溶活性的影响进而探讨其作用机制。结果不同相对分子质量岩藻聚糖硫酸酯可显著减轻血栓湿重,中相对分子质量岩藻聚糖硫酸酯比低相对分子质量表现出更强的抗血栓活性。低相对分子质量组(LF1组和LF2组)显著降低TXB2并同时升高6-keto-PGF1α;中相对分子质量组(MFa和MFb组)只降低TXB2,而对6-keto-PGF1α没有影响。4组岩藻聚糖硫酸酯均使t-PA活性升高、PAI-1活性降低、t-PA/PAI-1比值升高,但对PLG无影响。结论不同相对分子质量岩藻聚糖硫酸酯均有明显抗血栓作用,其作用机制可能与抗血小板聚集和纤溶系统活性升高有关,且随相对分子质量不同发挥抗血栓作用的主要机制不同。OBJECTIVE To study the antithrombotic activity of fucoidan with different molecular weight from Laminaria japoni- ca, and to explore its mechanism by observing antiplatelet activities and fibrinolysis. METHODS FeC13-induced arterial thrombosis in rats were used to examine the antithrombotie activity of four fucoidan fractions with different molecular weight; the TXB2 , 6-keto- PGF1 ct, PLG, t-PA and PAI-1 in plasma were determined by ELISA to evaluate the inhibitory effects of the drugs on platelet aggregation and fibrinolytic system. RESULTS Four fucoidan fraetions with different molecular weight could significantly decreased the weight of the thrombus, and Middle molecular weight （MMW） fucoidan fractions exhibited better antithrombotic activity than low molecular weight fucoidan （LMW）. Administration of LMW fucoidan significantly decreased the TXB2 content and promoted 6-keto-PGFlct content. Ad- ministration of MMW fucoidan only decreased the TXB2 content and didn＇t change 6-keto-PGFlct content. In four fucoidan fractions group, the activity of t-PA were increased, the activity of PAI-1 were decreased, the ratio of t-PA/PAI-1 was increased, and the activity of PLG had no change. CONCLUSION Four fucoidan fractions with different molecular weight have strong anti-thrombosis effect,the ac- tion mechanism may have correlation with the increase in the activities of anti-platelet aggregation and fibrinolysis system.

关 键 词：岩藻聚糖硫酸酯 抗血栓 组织型纤溶酶原激活物 纤溶酶原激活物抑制物 

分 类 号：R965[医药卫生—药理学]