整合素α_vβ_3对MDA-MB-231BO乳腺癌细胞AKT信号通路的调控  

Effect of Integrin α_vβ_3 on AKT Signaling Pathway of Breast Cancer MDA-MB-231BO Cells

作　　者：彭远远[1,2] 王捷[1] 夏冰[1] 杨传红[1] 冼江[1] 

机构地区：[1]广州军区广州总医院医学实验科,广州510010 [2]华南理工大学生物科学与工程学院,广州510006

出　　处：《生物技术通报》2012年第3期191-195,共5页Biotechnology Bulletin

基　　金：广东省自然科学基金项目(06104396)

摘　　要：旨在探究整合素αvβ3的单克隆抗体LM609在BSP不同表达水平的乳腺癌细胞中对AKT(蛋白激酶B)信号通路的影响。利用免疫细胞化学法检测BSP不同表达水平的乳腺癌细胞中整合素αvβ3的表达量。BSP基因沉默乳腺癌MDA-MB-231BO细胞,Western blotting在蛋白水平检测磷酸化AKT的表达,MTT试验和细胞划痕试验分别检测细胞增殖、迁移能力的变化。结果显示,与231BO-Scrambled细胞相比,231BO-BSP27细胞中BSP蛋白水平明显降低,抑制率达到(59.43±1.71)%;LM609分别处理两株细胞后,与对照组231BO-Scrambled细胞相比,BSP基因沉默组21BO-BSP27细胞中AKT磷酸化水平下调明显,为(33.78±1.51)%(P<0.01);231BO-BSP27细胞和对照组231BO-Scrambled中细胞的增殖和迁移能力均有不同程度的下降(P<0.05)。LM609能够抑制胞内整合素αvβ3功能的表达,进而对AKT信号通路进行调控,并影响细胞增殖和迁移的发生。It was to investigate the effect of integrin αvβ3 on the AKT signaling pathway in breast cancer MDA-MB-231BO cells.BSP-silencd MDA-MB-231BO cells were treated with integrin αvβ3-specific inhibitor LM609,Western blotting was used to detect AKT and phosphorylation of AKT,the proliferation of cells was analyzed by MTT assay and the migration of cells was analyzed by cell scratch assay.Results showed compared with control group 231BO-Scrambled cells,p-AKT protein expression in 231BO-BSP27 cells was significantly lower（33.78 ± 1.51）%（P〈0.01）.The result of MTT assay and cell scratch assay show significantly differences in 231BO-BSP27 and 231BO-Scrambled cells（P〈0.05）.LM609 can restrain the function of integrin αvβ3.Further regulate AKT signaling pathway of breast cancer MDA-MB-231BO cells,impact the proliferation and the migration of MDA-MB-231BO cells.

关 键 词：整合素ΑVΒ3 LM609 乳腺癌细胞 AKT信号通路 

分 类 号：R737.9[医药卫生—肿瘤]