人胃癌细胞株MGC-803侧群细胞的分选与生物学特征  

Isolation and biologic characteristics of the side population in human gastric cancer cell lines MGC-803

作　　者：俞薇[1] 吴婷[1] 司今[2] 姜伟[2] 李锦毅[2] 

机构地区：[1]辽宁医学院武警总医院研究生培养基地,北京100039 [2]武警总医院

出　　处：《山东医药》2012年第1期4-6,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81072969)

摘　　要：目的从人胃癌细胞株MGC-803中分离侧群(SP)细胞,研究其生物学特征。方法利用流式细胞分选术(FACS)从MGC-803细胞株中分选出SP细胞和非SP细胞亚群进行培养,采用克隆形成实验比较两组亚群细胞的体外增殖能力,NOD/SCID鼠成瘤实验检测两组亚群细胞体内成瘤能力。结果 FACS结果显示,细胞株MGC-803中SP细胞亚群占总细胞的0.3%～1.2%;SP细胞体外克隆形成率为(0.862±0.050)%,非SP细胞体外克隆形成率为(0.325±0.207)%,两者比较P<0.05;SP细胞最低成瘤数量是1×103/只,非SP细胞为5×103/只。结论人胃癌细胞株MGC-803中存在SP细胞,其生物学特性与干细胞基本符合。To investigate isolation and biologic characteristics of the side population（SP） in human gas- tric cancer cell lines MGC-803. Methods Fluoresence activated cell sorting was used to sort SP and NSP cells from MGC- 803s. Tumorigenicity of the two subpopulations was observed by sphere forming assay. Proliferation was determined by sub-cutaneous tumor formation in NOD/SCID mice. Results The percent of SP cells was 0.3% -1.2%. Clone formation effi-ciency of SP cells and NSP ceils was （ 0. 862 ± 0.050 ） % and （ 0. 325 ±0. 207 ） % respectively, there were significant differences （ P 〈 0.05 ）. In vivo, the tumorigenic ability of SP cells was higher than that of NSP cells. Tumor formed only by 1 × 10^3 SP cells in nude mice, but 5 × 103 NSP cells. Conclusion Human gastric cancer cell lines MGC-803 contained some SP cells with stem cell properties.

关 键 词：胃肿瘤 干细胞 分离 侧群细胞 

分 类 号：R735.2[医药卫生—肿瘤]