不同活性状态caspase-3在乳腺癌中的表达及意义  

Expression of caspase-3 in breast cancer and its significance

作　　者：王进京[1] 孙保存[1] 

机构地区：[1]天津医科大学病理学教研室,天津300070

出　　处：《山东医药》2012年第1期10-12,共3页Shandong Medical Journal

基　　金：国家自然科学基金重点项目(30830049);天津市科技计划重大攻关项目(09ZCZDSF04400)

摘　　要：目的检测不同活化形式的caspase-3在乳腺癌中的表达情况,探讨其在乳腺癌发生发展中的作用。方法采用免疫组织化学和原位杂交检测80例乳腺浸润性导管癌(IDC)、23例导管内癌(DCIS)、37例乳腺增生症和9例癌旁组织中caspase-3蛋白和mRNA表达、活性caspase-3表达,分析caspase-3与乳腺癌临床病理特征的关系。结果①caspase-3 mRNA在各组织中的表达无统计学差异(P>0.05)。caspase-3蛋白在IDC、DCIS、增生症中的表达均高于癌旁组织(P<0.05),IDC、DCIS均高于增生症组(P<0.05)。②活性caspase-3阳性细胞指数、pro-caspase-3阳性细胞指数在IDC、DCIS、增生症中均高于癌旁组织(P均<0.05),IDC、DCIS均高于增生症(P<0.05),IDC和DCIS无统计学差异(P>0.05),相同组织中的procaspase-3阳性细胞指数均明显高于活性caspase-3阳性细胞指数(P<0.05)。③caspase-3 mRNA与蛋白表达在导管内癌和增生症组织中存在相关性(P<0.05)。结论 caspase-3在乳腺癌中普遍阳性表达,但其并非以活性casapse-3的形式存在,提示caspase-3活化障碍可导致乳腺癌肿瘤细胞凋亡异常。To investigate the expression of caspase-3 in breast cancer and its role in tmnorigeuesis. Methods A total of 80 cases of breast invasive duct carcinoma（ IDC）, 23 cases of ductal carcinoma in situ（ DCIS）, 37 cases of cyclomastopathy and 9 cases of tumor adjacent tissues were selected. The expressions of mRNA and protein of caspase-3 and actived caspase-3 were investigated using in situ hybridization and immunohistochemistry. The clinical signif- icance of easpase-3 was evaluated. Results No significant differences of the expressions of caspase-3 mRNA were found among every group（P 〉0.05）. The expressions of easpase-3 protein in IDC, DCIS and cyclomastopathy were higher than those in tumor adjacent tissues（P 〈 0.05）. The expressions of caspase-3 protein in IDC and DCIS were higher than those in cyclomastopathy（P 〈 0.05 ）. The actived caspase-3 positive cell index（ac3i） and procaspase-3 positive cell index （pc3i） in IDC, DCIS and cyclomastopathy were higher than those in tumor adjacent tissues（P 〈 0.05 ）. The ac3i and pe3i in IDC and DCIS were higher than those in cyclomastopathy（P 〈 0.05）. No significant differences was found between IDC and DCIS（ P 〉 0.05 ）. The pc3i was higher than ac3i within every group （P 〈 0.05 ）. There were correlations between the expressions of caspase-3 mRNA and caspase-3 protein in DCIS and cyclomastopathy（P 〈 0.05）. Conclusions The expressions of caspase-3 protein in breast cancer are upregulated, but most of them are in the inactived form which indica- ted that failure of caspase-3 activation can lead to abnormal breast tumor cell apoptosis

关 键 词：乳腺肿瘤 细胞凋亡 CASPASE-3 

分 类 号：R737.9[医药卫生—肿瘤]