TRPV6对人卵巢癌细胞株HO-8910PM迁移的影响  

Effects of TRPV6 on the motility of HO-8910PM ovary cancer cells

作　　者：林丽琼 黄维甄[2] 陈健鹏[3] 

机构地区：[1]汕头市妇女儿童医院,广东汕头515041 [2]惠州市中心人民医院 [3]山东省立医院

出　　处：《山东医药》2012年第1期13-15,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81102052)

摘　　要：目的探讨TRPV6对卵巢癌细胞株HO-8910PM迁移能力的影响。方法细胞株分别瞬时转染TRPV6的特异性siRNA干扰序列,然后分别通过RT-PCR和Western-blot检测HO-8910PM细胞干扰前后TRPV6基因和蛋白表达变化,通过划痕试验和Transwell小室试验检测TRPV6对HO-8910PM细胞迁移的影响。结果 RT-PCR和Western-blot证实了TRPV6在人卵巢癌高转移细胞株HO-8910PM中的表达以及TRPV6 RNA干扰序列的干扰效率;划痕试验和Transwell小室试验表明,与HO-8910PM阴性干扰对照细胞相比,HO-8910PM-TRPV6-siRNA1和siR-NA2的迁移能力均显著下降(P<0.01)。结论卵巢癌细胞HO-8910PM中TRPV6的表达水平高低与该细胞迁移能力高低呈正相关,TRPV6有可能成为卵巢癌转移预防和治疗的新靶点。To investigate the effects of transient receptor potential V6 （TRPV6） channel on the motility of HO-8910PM ovary cancer cells. Methods After being permanently transfected with siRNA of the TRPV6 mRNA, TRPV6 mRNA and protein expressions were detected using reverse transcriptionPCR and western blot analysis, and the effect of TRPV6 on motility of HO-8910PM cell lines was detected by wound healing assay and Transwell chamber migration assay. Results RTPCR and Westernblot displayed that there were the expressions of TRPV6 gene and protein in HO-8910PM cells and the interference action of TRPV6 RNA interference sequential to HO-8910PM cells. Wound healing assay and Transwell chamber migration assay revealed that TRPV6 RNA interference significantly inhibited the migration of HO8910PMTRPV6-siRNA cells（ P 〈 0.01 ）. Conclusions TRPV6 has positive effects on the migration of HO-8910PM cells. TRPV6, therefore, might have potential prevention an treatment effects of ovary cancer as a new important targets.

关 键 词：卵巢肿瘤 瞬时受体电位 细胞运动 

分 类 号：R737.31[医药卫生—肿瘤]