副猪嗜血杆菌16S rRNA基因的克隆及序列分析  

Cloning and Sequence Analysis of 16S rRNA Gene for Haemophilus parasuis in Swine

作　　者：王祝荣 卢寄军 张进 

机构地区：[1]湖南省长沙市动物防疫监督站,湖南长沙410013

出　　处：《中国畜牧兽医》2012年第3期92-94,共3页China Animal Husbandry & Veterinary Medicine

基　　金：长沙科技计划一般项目(K0902144-21)资助

摘　　要：本研究旨在从分子水平对副猪嗜血杆菌湖南分离株进行鉴定,并用16SrRNA序列分析不同血型副猪嗜血杆菌之间的遗传关系。利用PCR扩增副猪嗜血杆菌的16SrRNA,应用ClustalX 1.81程序对序列进行比对,再用Phylip 3.67程序MP法和Mage 4.0程序NJ法绘制种系发育树,并用Puzzle 5.2程序构建最大似然树,同时利用DNAStar 5.0中的Megalign程序进行同源性分析。结果显示,所获得的16SrRNA序列长度均为783bp,湖南分离株与已知5型副猪嗜血杆菌位于同一分枝。结果表明,湖南分离株属于5型副猪嗜血杆菌,为副猪嗜血杆菌的分子流行病学和其相关疾病的诊断奠定基础。Haemophilus parasuis were identified by PCR from Hunan province isolates,and used 16S rRNA sequences to reconstruct their phylogenetic relationship.The partial 16S rRNA was amplified from each H.parasuis,and sequences were aligned using the ClustalX 1.81.MP and NJ trees of 16S rRNA were constructed using the software Phylip 3.67 version 4.0 and Mega version 4.0,and ML tree was also constructed using Puzzle version 5.2.Sequence homology analysis was performed using the Megalign program of the software DNAStar version 5.0.The results showed that the lengths of 16S rRNA sequences were 783 bp.The constructed phylogenetic tree revealed that the Hunan isolates and the H.parasuis serovar 05 available in GenBank were clustered in the same clade.The results of the present study provided foundation for further studies of molecular epidemiology of H.parasuis and for diagnosis of the resultant disease.

关 键 词：副猪嗜血杆菌 16S RRNA 种系发育关系 

分 类 号：Q78[生物学—分子生物学]