Myostatin在地塞米松致肌原纤维损伤中的作用研究  

Research on the Role of Myostatin in Dexamethasone-induced Myofibril Damage

作　　者：秦健[1] 杜荣[2] 杨亚群[2] 周英[2] 张红强[2] 刘文花[2] 

机构地区：[1]山西农业大学信息科学与工程学院,太谷030801 [2]山西农业大学动物科技学院,太谷030801

出　　处：《畜牧兽医学报》2012年第3期482-488,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30771580;31140074);教育部科学技术研究重点项目(210027);山西省自然科学基金(2008011057-2);山西省高等学校优秀青年学术带头人支持计划;山西农业大学中青年拔尖创新人才支持计划

摘　　要：为阐明Myostatin在肌原纤维损伤中的作用,并探讨提高畜禽肉产(质)量或控制人类肌肉萎缩的方法,本试验以小鼠为模式动物,采用高剂量地塞米松构建了严重应激模型,研究生理剂量胰岛素对地塞米松致肌原纤维损伤的影响及其与Myostatin基因表达的关系,以及Myostatin基因免疫对地塞米松致肌原纤维损伤的干预作用。结果表明:地塞米松诱导Myostatin基因表达上调及严重的肌原纤维损伤和线粒体肿胀,而胰岛素注射明显减弱了这些效应;Myostatin基因免疫明显抑制了地塞米松对肌原纤维和线粒体的损伤作用。试验结果提示,Myostatin是参与肌原纤维降解的一个关键因子,该作用可能与其刺激了线粒体的功能有关。In order to clarify the roles of myostatin in myofibril damage and investigate the meas ures to improve the production and quality of muscle mass in livestock or control the muscle atro- phy in human, the serious stress models of mice were made with high-dose dexamethasone, and the effect of physiological-dose insulin on the dexamethasone-induced myofibril damage and the relationship between the effect and myostatin expression were researched. In addition, the inter- vention effect of myostatin gene immunization on the dexamethasone-induced myofibril damage was studied. The results showed that. dexamethasone upregulated the expression of myostatin gene and caused the serious damage of myofibril and swelling of mitochondria, while insulin at tenuated the effects of dexamethasone~ myostatin gene immunization reduced the myofibril and mitochondria damages induced by dexamethasone. The results suggest that myostatin is one of key factors for myofibril degradation and the role of myostatin may be attributed to the stimula- tion of mitochondrial function by myostatin.

关 键 词：MYOSTATIN 肌原纤维 地塞米松 胰岛素 基因免疫 线粒体 

分 类 号：S852.33[农业科学—基础兽医学]