可诱导肿瘤靶向性IFN-α2a重组腺病毒的构建及其表达  

Construction and Expression of the Recombinant Inducible Tumor-targeted Adenovirus Containing IFN-α2a

作　　者：李祥[1] 璩津生[1] 康帅[1] 何哲耘[1] 杨纪峰[1] 高基民[1] 

机构地区：[1]温州医学院生命与检验学院,温州325035

出　　处：《中国细胞生物学学报》2012年第3期265-271,共7页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(No.30900344);浙江省自然科学基金(No.Y2080676);温州医学院本专科学生科研立项基金(No.wyx201001005)资助项目~~

摘　　要：腺病毒载体广泛应用于恶性肿瘤的靶向性基因治疗的研究,但这些肿瘤靶向载体缺乏可控性,其疗效和安全性受到很大的影响,因此开发新型可诱导的生物肿瘤靶向载体是当今抗肿瘤靶向药物研究的当务之急。该研究构建了可诱导肿瘤靶向性IFN-α2a重组腺病毒。重组腺病毒能够有效感染人肝癌细胞株HepG2等多种肿瘤细胞株,RT-PCR和Western blot结果表明肿瘤细胞能高效表达IFN-α2a,而其非肿瘤细胞株L02几乎没有表达。诱导试验表明重组腺病毒Ad MT-Ⅱ-hTERT/IFN-α2a能被ZnSO4诱导表达。可诱导肿瘤靶向性重组腺病毒Ad MT-Ⅱ-hTERT/IFN-α2a成功构建为下一步体内外抑癌实验研究打下了基础。Adenovirus-based vector,which is one of the widely used gene transfer tools,especially applied in metastatic tumor gene therapy,shows a very promising prospect.However,these tumor-targeted vectors lack of controllability,and their efficacy and safety are greatly affected.Therefore,the development of new inducible tumor-targeted recombinant vectors has attracted much attention in recent years.Here,we construct the inducible tumor-targeted recombinant adenovirus containing IFN-α2a.The recombinant adenovirus can infect many tumor cell strains such as HepG2 cells efficiently.RT-PCR and Western blot results showed that the IFN-α2a gene expressed specifically in tumor cells,while hardly expressed in non-tumor cells such as L02.After induction with ZnSO4,the expression levels of IFN-α2a are higher than those no inducted.The inducible tumor-targeted recombinant adenovirus containing IFN-α2a can be further studied for inhibition effect on cancer in vitro and in vivo.

关 键 词：肿瘤靶向性 腺病毒 HTERT启动子 MT-Ⅱ启动子 

分 类 号：R730.2[医药卫生—肿瘤]