海南广藿香叶和茎挥发油化学成分的气相色谱-质谱联用分析  

GC-MS Analysis of Essential Oil in Leaves and Stems of Pogostemon Cablin Collected from Hainan Province

作　　者：吴友根[1,2] 郭巧生[1] 梁振益[3] 范春蕾[3] 张军锋[3] 

机构地区：[1]南京农业大学中药材研究所,江苏南京210095 [2]热带作物种质资源保护与开发利用教育部重点实验室海南大学园艺园林学院,海南海口570228 [3]海南大学材料化工学院,海南海口570228

出　　处：《时珍国医国药》2012年第3期527-530,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30960533);海南省自然科学基金项目(No.310022);海南省教育厅高等学校科学研究资助项目(No.Hjkj2009-25);海南大学科研资助项目(No.hd09xm58);海南大学科研启动基金项目(No.kyqd1002)

摘　　要：目的研究超临界CO2流体萃取和水蒸气蒸馏提取法提取海南广藿香叶和茎中挥发油,鉴定各化学组分并测试其含量,为海南广藿香指纹图谱的构建和质量标准的建立奠定基础。方法采用GC-MS技术和标准图谱检索对照、核对和补充检查的方法分离和鉴定各化学组分,用离子流色谱峰面积归一法计算各化学成分的相对含量。结果从水蒸气蒸馏提取的叶挥发油中分离出40个色谱峰,鉴定了35个化合物,占总量的97.82%,含量在1%以上的成分有16个,以百秋李醇含量最高,为24.32%;从水蒸气蒸馏提取的茎油中分离得到17个色谱峰,全部被鉴定。其中12种化合物的含量超过1%,亦以百秋李醇含量最高,为33.32%;从超临界CO2萃取的叶挥发油中分离出39个色谱峰,全部被鉴定,其中含量在1%以上的成分有16个;从超临界CO2萃取的茎油中分离得到24个色谱峰,全部被鉴定,15种化合物的含量超过1%,以百秋李醇含量最高,为22.06%。结论鉴定的化合物主要为薁(甘菊环)、烯、酚、酯、醇、醛、酮、萘、烷、酸类等成分,其中百秋李醇的含量较高。Objective To analyze and identify the chemical components and relative contents of essential oil extracted by supercritical-CO2 fluid extraction（SFE） and steam distillation（SD） from P.cablin. Methods By using GC-MS techniques,referring to the search of standard spectrum and calculating the relative contents of the chemical compositions with the method of ionic peak area. Results Thirty-five and seventeen compounds were identified in the essential oil of leaves and stems extracted by SD,respectively.There were sixteen and twelve compounds whose relative contents were over 1%,respectively.The relative contents of patchouli alcohol was the most compound in the essential oil of leaves and stems,were 24.32% and 33.32%,respectively.Thirty-nine and twenty-four compounds were identified in the essential oil of leaves and stems extracted by SFE,respectively.There were sixteen and fifteen compounds whose relative contents were over 1%,respectively.The relative content of patchouli alcohol,the most compound in the essential oil of stems,was 22.06%. Conclusion The major compounds are azulene,eudesma,hydroxybenzene,ester,alcohol,aldehyde,ketone,naphthaline,alkyl and acid,and the relative contents of patchouli alcohol in all essential oils are higher than those of other compounds.

关 键 词：广藿香 叶和茎 挥发油 气相色谱-质谱联用技术 

分 类 号：R284.2[医药卫生—中药学]