江西道地药材车前草中总黄酮超声辅助提取研究  

Ultrasonic-assisted Extraction of Total Flavonoid from Plantago asiatica L.

作　　者：童声[1] 范杰平[1] 徐薇[1] 田泽由[1] 段勇华[1] 孔涛[1] 张璐[1] 

机构地区：[1]南昌大学环境与化学工程学院鄱阳湖环境与资源利用教育部重点实验室,江西南昌330031

出　　处：《时珍国医国药》2012年第3期553-555,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.20806037);江西省教育厅自然科学基金(No.GJJ09420);江西省研究生创新专项资金(No.YC09B012);南昌大学"鄱阳湖环境与资源利用"教育部重点实验室开放基金资助(No.Z04998)

摘　　要：目的探讨超声辅助提取车前草中总黄酮的工艺。方法在单因素实验基础上,采用正交实验设计法对提取工艺进行优化。结果各因素对提取率影响大小依次为:超声提取时间(A)>液固比(C)>乙醇浓度(B);最佳条件为:在20℃下,用40%乙醇为溶剂,液固比8∶1,超声提取20 min。在此条件下,提取两次,总黄酮能基本被提取完全,总提取率可达0.371%。结论该方法采用超声波辅助强化提取,工艺简单可行,达到省时、高效和节能的目的。Objective To optimize the ultrasonic-assisted extraction of total flavonoid from Plantago asiatica L.. Methods On the basis of single-factor experiments,the optimal extraction conditions of total flavonoid were investigated with orthogonal experimental design. Results The significance of these factors affecting the total flavonoid was in the following order： ultrasonic-assisted extraction time liquid/solid ratio ethanol concentration.The optimal parameters were as follows： ethanol concentration of 40%,ratio of solvent to solid of 8：1,extraction time of 20 min at 20 ℃.Under the optimal conditions,after two times of extraction the total extraction yield was 0.371%. Conclusion The optimal method is reliable,reasonable and effective.

关 键 词：车前草 总黄酮 超声辅助提取 

分 类 号：R284.2[医药卫生—中药学]