几种理化因子对斑节对虾白斑杆状病毒(WSBV)感染力的影响  

EFFECT OF SEVERAL PHYSICAL AND CHEMICAL FACTORS ON VIABLITY OF WHITE SPOT BACULOVIRUS OF Penaeus monodon

作　　者：谢数涛[1] 邱德全[2] 何建国[1] 杨晓明[1] 江静波[1] 

机构地区：[1]中山大学生命科学学院,广州510275 [2]湛江海洋大学水产学院,524088

出　　处：《海洋科学》2000年第3期52-54,30,共4页Marine Sciences

基　　金：国家自然科学基金资助项目!39670577号

摘　　要：将部分纯化的WSBV暴露于不同理化条件下 ,通过人工感染的方法测定WSBV的感染力。结果表明 ,病毒在纯水中1h能保持感染活性,但在3mol/LNaCl的高盐溶液中1h失去感染力 ;WSBV在30℃中4h感染活性丧失 ,在70～90℃中10min均失去感染力 ;当环境 pH值为5以下或12.6以上时 ,WSBV在1h后丧失感染活性 ;NP 40,TritonX 100,ClO2Stability of WSBV to NaCl concentration, pH, heat and other chemical was investigated using clinical health Penaeus monodon in a bioassay. WSBV was completely inactivity within one hour after exposure to a solution of 3 mol/L NaCl. It lost infectivity after exposure to 30 ℃ for 4 h or 70 ℃ for 5 min. When the pH value of solution was higher than 12,6 or lower than 5.0, WSBV was completely inactivated within 1 h. So WSBV seemed to be more sensitive to acid solution than to basic solution. WSBV lost infectivity after exposure to NP 40, Triton X 100, ClO2 or formalin more than 1 h.

关 键 词：白斑杆状病毒 斑节对虾 理化因子 感染力 虾病 

分 类 号：S945.27[农业科学—水产养殖]