反义CD44基因转染对人眼小梁细胞合成细胞外基质的影响  

Effect of CD44-specific antisense oligonucleotide on extracellular matrix synthesis of human trabecular meshwork cells

作　　者：李中国[1] 王青[2] 梅红英[2] 罗一青[2] 陈永娟[2] 张宇宏[2] 余萍[2] 云亚歌[2] 史强[2] 

机构地区：[1]南京医科大学附属南京医院(南京市第一医院)眼科,中国江苏省南京市210006 [2]青海大学附属医院眼科,中国青海省西宁市810001

出　　处：《国际眼科杂志》2012年第4期615-617,共3页International Eye Science

基　　金：中国青海省自然科学基金资助项目(No.2009-Z-706)~~

摘　　要：目的:观察反义CD44基因转染对人眼小梁细胞合成细胞外基质的影响,探讨黏附分子CD44在原发性开角型青光眼(primary open angle glaucoma,POAG)发病过程中可能的作用。方法:采用硫代修饰的CD44反义寡核苷酸,通过脂质体介导转染体外培养的人眼小梁细胞,免疫组织化学染色观察CD44反义寡核苷酸对小梁细胞合成胶原蛋白Ⅰ型、层黏附蛋白的影响,放射免疫法观察CD44反义寡核苷酸对小梁细胞合成透明质酸的影响。结果:CD44反义寡核苷酸对人眼小梁细胞合成胶原蛋白Ⅰ型、层黏附蛋白起促进作用,且呈浓度依赖性,浓度越高促分泌作用越强,但对小梁细胞合成透明质酸起抑制作用,浓度越高抑制作用越明显。结论:CD44反义寡核苷酸封闭CD44基因表达后人眼小梁细胞合成胶原蛋白Ⅰ型、层黏附蛋白增加而合成透明质酸减少。黏附分子CD44可能通过影响小梁细胞合成细胞外基质功能参与了POAG发病过程。AIM：To observe the effect of CD44-specific antisense oligonucleotide on extracellular matrix（ECM） synthesis of human trabecular meshwork cells,then to discuss the possible relationship between CD44 and primary open angle glaucoma（POAG）.METHODS：CD44-specific antisense oligonucleotide were delivered with cationic lipid to cultured human trabecular meshwork cells.Effect of CD44-specific antisense oligonucleotide on synthesis of collagen-Ⅰ,Laminin were examined by immunohistochemistry and the synthesis of hyaluronic acid were examined by radioimmunoassay.RESULTS：Three different concentrations of CD44 antisense oligonucleotide could promote the synthesis of collagen-Ⅰ,Laminin in trabecular meshwork cells and in a concentration-dependent manner.But CD44 antisense oligonucleotide inhibited the synthesis of hyaluronic acid.CONCLUSION：CD44 antisense oligonucleotide promotes the synthesis of collagen-Ⅰand Laminin but inhibits the synthesis of hyaluronic acid.CD44 may play a role in pathogenesis of POAG by affecting the synthesis of ECM.

关 键 词：小梁细胞 CD44 反义寡核苷酸 细胞外基质蛋白 

分 类 号：R775[医药卫生—眼科]