瘢痕成纤维细胞α_(1-4)整合素表达研究  

Expression of α_(1-4) integrins in hypertrophic scar fibroblasts

作　　者：赵烨德 何清濂[2] 牛星焘[3] 

机构地区：[1]上海海军第四一一医院整形科,200081 [2]上海长征医院整形科 [3]北京医科大学第三医院整形科

出　　处：《中华整形外科杂志》2000年第1期37-39,共3页Chinese Journal of Plastic Surgery

摘　　要：目的　了解增生性瘢痕组织内成纤维细胞膜上α1 4整合素粘附分子表达水平 ,并探讨整合素在瘢痕增生发展过程中的作用及意义。方法　提取增生性瘢痕及正常皮肤组织中的细胞膜蛋白 ,以免疫斑点杂交检测整合素表达水平 ;组织标本石蜡切片 ,以免疫组织化学染色方法显示不同亚型整合素的表达部位。结果　①从增生性瘢痕组织中提取的膜蛋白内含有相对较高的α1、α2 、α3和α4整合素成份 ;②在正常皮肤及增生性瘢痕组织的表皮基底细胞膜上均有不同程度的α1 3整合素表达 ,但正常皮肤成纤维细胞则无整合素阳性信号发现 ,而在增生性瘢痕组织内的成纤维细胞膜上则有着不同程度的α1 4整合素表达 ,尤其以瘢痕胶原结节内及接近正常组织部位最为明显。结论增生性瘢痕组织内成纤维细胞膜上α1 4整合素粘附分子的过度表达说明其具有比正常成纤维细胞更强的结合细胞外间质能力 ,同时细胞内外信号之间的传递更为广泛、紧密及迅速 ;因此 ,我们认为整合素粘附分子在瘢痕成纤维细胞膜上的过度表达不但对于该细胞发挥异常的生物学活性有着积极的调节作用 ,同时对于瘢痕结缔组织重建有着重要影响。Objective To determine the expression levels of α 1 4 integrins in hypertrophic scar fibroblasts and investigate the role that α 1 4 integrins may play in the process of hypertrophic scar formation.Methods The membrane protein was extracted from hypertrophic scar and normal skin tissues.The content of α 1 4 integrins of the membrane protein was detected by immunoblotting method.The locations of α 1 4 integrin expression were examined by immunohistochemical stain.Results ①Higher concentration of α 1 4 integrins was identified in the membrane protein extracted from hypertrophic scar than from normal skin.②In normal skin,the expression of α 1, α 2 and α 3 integrins located on the epidermal cell surface but not on the dermal fibroblast surface.In the hypertrophic scar,not only did the epidermal cell surface express α 1 3 integrins,but also the fibroblast surface expressed α 1 4 integrins.Conclusions The expressions of hypertrophic scar fibroblast surface integrins are subjected not only to cell extracellular matrix adhesion,but also to intracellular/extracellular signal transduction.Integrins may play an important role in regulating much of fibroblast behavior,including growth,differentiation,phenotype,and in scar tissue remodelling.

关 键 词：整合素 瘢痕 成纤维细胞 细胞外间质 

分 类 号：R363[医药卫生—病理学]