B7瘤苗与DC瘤苗诱导的抗L615白血病的免疫作用  

B7 and DC vaccines induced anti tumor immunity against murine T-lymphocyte leukemia L615

作　　者：李牧[1] 尤胜国[1] 马楠[1] 廖晓龙[1] 魏代奎 葛薇[1] 马双[1] 

机构地区：[1]中国医学科学院

出　　处：《中华血液学杂志》2000年第1期20-22,共3页Chinese Journal of Hematology

基　　金：国家自然科学基金!3 93 70 770;天津市自然科学基金!963 60 1911

摘　　要：目的　研究B7瘤苗和负载了肿瘤细胞膜抗原的DC瘤苗在小鼠L6 15白血病模型中诱导抗白血病免疫的作用。方法　通过观察小鼠的存活率和生存时间 ,研究两种瘤苗在动物体内诱导免疫预防保护的能力 ;通过体外细胞毒实验和细胞增殖实验 ,检测两种瘤苗在体外诱导的T细胞特异性杀伤活性及对特异性抗原刺激的增殖活性。结果　在体内 ,两种瘤苗免疫的小鼠对随后活瘤细胞的攻击均有保护作用 ,DC瘤苗效果更佳 ;同时还发现输注免疫保护鼠的DC对荷瘤小鼠有明显的治疗效果。在体外 ,两种瘤苗均可诱导出对肿瘤细胞的特异性杀伤活性和对抗原的特异性增殖活性 ,DC瘤苗的活性更显著。结论　DC瘤苗以其安全、有效、易行的特点 ,将会在肿瘤免疫治疗中具有良好的应用前景。Objective To study the effect of B7 and DC vaccines in inducing anti tumor immunity in murine T lymphocyte leukemia L615.Methods In vivo,murine mortality and survival were observed to compare the difference between B7 and DC vaccine in inducing immunoprotection against the subsequent challenge of live L615 cells;in vitro, specific cytotoxic assay and MLR were performed to test the specific cytotoxicity and proliferative activity of effective T cells.Results Both vaccines could efficiently improve T cell mediated anti leukemic immunity in syngenic hosts,and DC vaccine was revealed to be more efficient.In vitro observations showed that both vaccines could induce tumor specific cytotoxicity and proliferative activity of effective T cells.Conclusion DC vaccine is promising in tumor immunotherapy owing to its safety,efficiency and convenience.

关 键 词：癌症疫苗 树突细胞 白血病 B7瘤苗 

分 类 号：R733.7[医药卫生—肿瘤]