上调SMYD3对人胆管癌FRH0201细胞中DNMT3B表达及细胞增殖的影响  

Effect of SMYD3 over-expression on DNMT3B levels and proliferation ability in human cholangiocarcinoma cell line FRH0201

作　　者：程帝[1] 李志花[1] 陈汝福[2] 郭宁[2] 廖巧芳[1] 郑礼平[1] 周泉波[2] 周嘉嘉[2] 

机构地区：[1]中山大学孙逸仙纪念医院肿瘤科,广东广州510120 [2]中山大学孙逸仙纪念医院肝胆外科,广东广州510120

出　　处：《中国病理生理杂志》2012年第3期415-419,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30872485)

摘　　要：目的:研究人胆管癌细胞株FRH0201中SET和MYND结构域含有蛋白3(SET and MYND domain-containing protein 3,SMYD3)过度表达对DNA甲基化转移酶3B(DNA methyltransferase 3B,DNMT3B)表达及细胞增殖能力的影响。方法:瞬时转染SMYD3真核表达质粒后,RT-PCR检测细胞中DNMT3B mRNA水平的变化;Western blotting检测细胞中DNMT3B蛋白水平的变化;CCK-8检测细胞增殖能力的改变;流式细胞术检测细胞周期的改变。结果:以未处理组为对照,胆管癌细胞FRH0201在转染pEGFP-C3-SMYD3质粒后,DNMT3B蛋白及mRNA表达均显著上升(P<0.01);细胞的增殖能力显著提高、细胞增殖速度加快(P<0.05);进入G_2/M期的细胞明显增多(P<0.05)。结论:过度表达SMYD3,可引起细胞中DNMT3B的表达上调并增强细胞增殖能力。AIM：To explore the effect of SET and MYND domain-containing protein 3（SMYD3 ） over-expression on the expression of DNA methyltransferase 3B（DNMT3B） and the proliferation ability in human cholangiocarcinoma cell line FRH0201.METHODS：Transient transfection of SMYD3 eukaryotic expression plasmid pEGFP-C3-SMYD3 into human cholangiocarcinoma cell line FRH0201 was performed.The expression of DNMT3B at mRNA and protein levels was detected by RT-PCR and Western blotting,respectively.Cell proliferation was examined by CCK-8 method and cell cycle situation was checked by flow cytometry.RESULTS：After transfected with SMYD3 eukaryotic expression plasmid pEGFP-C3-SMYD3,the over-expression of SMYD3 in FRH0201 cells was observed.Compared with the untransfected cells,the expression of DNMT3B was significantly increased（P〈0.01）,the proliferation rate was obviously accelerated（P〈0.05） and the number of the cells in G_2/M phase was significantly increased（P〈0.05） in FRH0201 cells transiendy transfected with pEGFP-C3-SMYD3 plasmid.CONCLUSION：The transient transfection of pEGFPC3-SMYD3 plasmid induces over-expression of DNMT3B and promotes the proliferation of human cholangiocarcinoma cell line FRH0201.

关 键 词：FRH0201细胞 甲基化 组蛋白甲基化转移酶 DNA甲基化转移酶 

分 类 号：R735.8[医药卫生—肿瘤]