纳米羟基磷灰石材料复合碱性成纤维细胞因子促进牙周组织再生的实验研究  

Periodontal regeneration enhanced by the application of nano-Hap/collagen and basic fibroblast growth factor

作　　者：鲁红[1] 田宇[1] 吴织芬[1] 王小勇[2] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032 [2]军事科学院门诊部,北京100091

出　　处：《牙体牙髓牙周病学杂志》2012年第3期128-131,163,共5页Chinese Journal of Conservative Dentistry

基　　金：陕西省科学技术研究发展计划项目(2010K12-02-03;2010K01-193)

摘　　要：目的:探讨新型纳米羟基磷灰石材料—纳米羟晶/胶原仿生骨材料(nHAC)作为生长因子载体和牙周组织工程支架材料应用的可行性,观察评价其对牙周组织再生修复的影响和意义。方法:将人牙周膜细胞(HPDLCs)接种于nHAC三维支架上复合培养,体外扫描电镜观察HPDLCs在nHAC支架上的附着、生长情况,并将碱性成纤维细胞生长因子(bFGF)与nHAC复合植入动物人工牙周组织缺损,表面覆盖聚四氟乙烯(e-PTFE)膜,以空白对照组和单纯置膜组作为对照。术后8周进行组织学观察和测量,分析评价其牙周组织的再生情况。结果:扫描电镜可见nHAC具有良好的多孔网状结构,PDLCs在nHAC支架上贴附紧密,生长旺盛,伸展充分,动物实验结果显示nHAC/bFGF/膜复合植入组较空白对照组和单纯置膜组有更多新生的牙槽骨、牙周膜和牙骨质生成,结果具有显著性差异。结论:nHAC具有良好的三维空间结构和细胞相容性,与bFGF复合植入牙周缺损后可显著促进牙周组织再生,提示nHAC有望成为理想的生长因子载体和牙周组织工程支架材料。AIM： To evaluate the feasibility of nano-Hap/collagen（nHAC） as carrier of growth factors and scaffold material for periodontal tissue engineering,and to observe and evaluate the effects of nHAC and basic fibroblast growth factor（bFGF） on periodontal regeneration.METHODS： Periodontal ligament cells（PDLCs） cultured in vitro were collected and seeded on three-dimensional framework of nHAC.The cell growth in the scaffolds was observed by scanning electron microscopy.The three-dimensional frameworks of nHAC combined with bFGF were transplanted into man-made periodontal defects covered with e-PTFE membranes.And the groups covered with nothing or only with membranes were the controls.The dogs were sacrificed after 8 weeks and the periodontal regeneration was observed histologically.RESULTS： Scanning electron microscope showed the porous structure of nHAC and the eugenic growth of cells in the scaffolds.And the histological results showed that the bFGF/nHAC/membrane groups exhibited more new bone,new periodontal ligament and new cementum occupying the majority of the defects than the control groups,and there were statistical differences.CONCLUSION： The combined application of nHAC and bFGF can contribute to enhancement of periodontal regeneration,and nHAC is valued to further study as carrier of growth factors and scaffolds of periodontal tissue engineering.

关 键 词：组织工程 支架材料 纳米羟基磷灰石 碱性成纤维细胞生长因子 

分 类 号：R780.2[医药卫生—口腔医学]