慢性牙周炎龈组织中Smac和Bax的表达及其对细胞凋亡的作用  

Expression of Smac and Bax in chronic periodontal tissues and their roles in apoptosis

作　　者：冯利[1] 董跟喜[2] 孙晓玮[1] 

机构地区：[1]兰州大学基础医学院,甘肃兰州730000 [2]兰州大学口腔医学院,甘肃兰州730000

出　　处：《牙体牙髓牙周病学杂志》2012年第3期132-135,共4页Chinese Journal of Conservative Dentistry

基　　金：甘肃省自然科学基金(0803RJZA053)

摘　　要：目的:通过检测Smac和Bax蛋白在慢性牙周炎龈组织中的表达,旨在探讨二者的相关性及其在细胞凋亡中与牙周炎发生、发展的关系。方法:慢性牙周炎测试组27人,健康对照组27人,利用光镜和透射电镜观察凋亡细胞形态结构,流式细胞仪测定线粒体膜电位。免疫组化检测Smac、Bax在龈组织不同区域的表达。结果:光镜观察慢性牙周炎龈组织中存在有凋亡细胞;电镜观察凋亡细胞中线粒体形态结构改变;慢性牙周炎龈组织线粒体膜电位降低,与健康组比较,两组间有显著性差异(P<0.05);慢性牙周炎组中Smac和Bax在上皮组织、结缔组织的表达增加,与健康组相比具有显著性差异(P<0.05);Smac、Bax二者的表达在慢性牙周炎龈组织具有明显正相关(P<0.05)。结论:慢性牙周炎龈组织中有凋亡细胞存在;Smac、Bax的表达在慢性牙周炎组中呈明显正相关,表明在细胞凋亡过程中起协同作用,这与牙周组织破坏有关。AIM： To investigate the expression of Smac and Bax in gingival tissues of chronic periodontitis patients.METHODS： Gingival tissues from 27 periodontitis patients and 27 periodontally healthy subjects were obtained from extracted teeth.Light microscope and transmission electron microscope were used to observe the morphology and structure of apoptotic cells in gingival tissues.Mitochondrial transmembrane potential（△Ψm） was measured by flow cytometry.The expression of Smac and Bax was detected with immunohistochemistry in gingival tissues.RESULTS： Under light microscope,apoptotic cells could be observed.Morphological and structural changes could be seen in mitochondria under transmission electron microscope.△Ψm was significantly lower in chronic periodontal tissues as compared with healthy gingiva（P0.05）.Smac and Bax expression was significantly stronger in gingival epithelial and connective tissues of chronic periodontitis patients（P0.05）.Smac expression was positively related to Bax expression（P0.05） in chronic periodontal tissues.CONCLUSION： Apoptotic cells exist in gingival tissue of chronic periodontitis patients.Smac expression is positively related to Bax expression.Both Smac and Bax expression are significantly enhanced,indicating that they might play important roles in the destruction of periodontal tissues.

关 键 词：慢性牙周炎 Smac和Bax 细胞凋亡 

分 类 号：R780.2[医药卫生—口腔医学]