内切中性纤维素酶EGⅡ的发酵条件优化  

Optimization of Fermentation Conditions for Neutral Endoglucanase EGⅡ

作　　者：郑海英[1] 蔡少丽[1] 黄平[1] 杨威[1] 黄杨[1] 杨鹤[1] 张爱平[1] 刘小琳[1] 黄建忠[1] 

机构地区：[1]福建师范大学工业微生物教育部工程研究中心生命科学学院福建省现代发酵技术工程研究中心,福建福州350108

出　　处：《微生物学杂志》2012年第1期12-16,共5页Journal of Microbiology

基　　金：福建省发改委产业化关键技术项目(闽发改投资[2009]958号)

摘　　要：对毕赤酵母基因工程菌EIM-50-eg2产内切中性纤维素酶的主要影响因子进行研究,考察氮源、pH、温度、微量元素PTM1和甲醇浓度等对工程菌产酶的影响。单因素实验和正交实验结果表明,优化后的培养基组成及培养条件:磷酸氢二铵40 g/L,甲醇15 mL/L,硫酸镁10 g/L,磷酸二氢钾9 g/L,初始pH 6.0,培养温度28℃,PTM1添加量0.02%,甲醇诱导浓度1.5%。优化后内切葡聚糖酶活力可达4 158 U/(mL.min)是优化前1 449 U/(mL.min)的2.86倍。The main influencing factors of neutral endoglucanase produced by genetic engineered Pichia pastoris EIM- 50-eg2 were studied, and investigated the effects of nitrogen source, pH, temperature, PTM1 concentration, methanol concentration on the enzyme production of the engineered strain. The single factor and orthogonal design experiment results showed that the optimized fermentation conditions were： （NH4 ）2HPO4 40 g/L, CH3OH 15 mL/L, MgSO4 10 g/L, KH2PO4 9 g/L, with initial at pH 6.0, cultured at 28 ℃ , PTM1 0.02% and methanol concentration 1.5%. Under the optimal condition the activity of endoglucanase reached at 4 158 U/（mL . min） , which was 2.86 times as compared with 1 449 U/（mL . min） before the optimization.

关 键 词：内切中性纤维素酶 毕赤酵母 优化 

分 类 号：Q93[生物学—微生物学]