RRLC测定发酵液中达托霉素的含量  

RRLC Determination of Daptomycin in Fermentation Broth

作　　者：周剑[1] 谢颖[1] 黄建峰[1] 郑卫[1] 方东升[1] 

机构地区：[1]福建省微生物研究所福建省新药(微生物)筛选重点实验室,福建福州350007

出　　处：《福建分析测试》2012年第1期46-49,共4页Fujian Analysis & Testing

基　　金：福建省自然科学基金资助(项目编号2011J01094)

摘　　要：建立可快速准确测定玫瑰孢链霉菌发酵液中达托霉素含量的RRLC方法。采用高分离度快速液相色谱(RRLC)Agilent 1200 series,色谱柱:Aglient XDB C18,1.8u,4.6*50mm。流动相:0.1%三氟乙酸水溶液:乙腈=64:36,检测波长为214 nm,柱温为30℃。结果:达托霉素浓度范围在25～500ug/ml线性良好,相关系数为0.9989;测得发酵液样品的平均回收率为101.7%,RSD为0.63%。结论:方法准确可靠,可以用于达托霉素发酵过程的含量测定。Object：Develop a RRLC method for the determination of daptomycin in fermentation broth of Streptomycesroseosporus. Methods： Using RRLC Agilent 1200 series, Aglient XDB C18 （1.8u, 4.6＊50mm）, the mobile phase was 0. 1% Trifluoroacetic acid （TFA） ：acetonitrile=64：36 at a flow rate of 1.0mL.min-land detection wavelength was 214 nm, column temperature was 30 ℃. Results ： The calibration curve was linear over the range of 25-500ug/ml with the correlation 0.9989 （n=7）. The average recovery of daptomycin was 101.7% （RSD=0.63 % ）. Conclusion： The method is accurate, simple with good reproducibility, which is suitable for the determination of daptomycin in fermentation broth.

关 键 词：达托霉素 发酵液 高分离度快速液相色谱(RRLC) 

分 类 号：R917[医药卫生—药物分析学]