血红素氧化酶1可提高脐血间充质干细胞移植梗死心肌细胞的存活率  

Heme oxygenase-1 can improve the survival rate of canine cord blood-derived mesenchymal stem cells transplanted into infarcted hearts

作　　者：陈德海[1] 王萍[1] 张申[1] 吴联合[1] 张培喜[1] 马南[2] 

机构地区：[1]济宁市第一人民医院胸心外科,山东省济宁市272111 [2]上海交通大学医学院附属新华医院胸心外科,上海市200092

出　　处：《中国组织工程研究》2012年第6期1024-1027,共4页Chinese Journal of Tissue Engineering Research

基　　金：济宁市科委课题项目(2009-56-2-9)~~

摘　　要：背景:血红素氧化酶1具有较强的内源性抗氧化作用,可减轻炎症反应,发挥细胞保护作用。目的:观察血红素氧化酶1对犬脐血间充质干细胞梗死心肌移植后细胞存活率的影响。方法:取第4代体外培养脐血间充质干细胞,用含氯化高铁血红素的培养液培养24h,免疫组织化学法检测细胞血红素氧化酶1的表达;将细胞经LacZ报告基因标记后经冠状动脉植入犬心肌梗死区域。结果与结论:经含氯化高铁血红素培养液培养后的脐血间充质干细胞血红素氧化酶1表达明显上调,并持续2周以上;移植犬梗死心肌区后可长期存活,存活能力明显增强。结果表明氯化高铁血红素可诱导上调犬脐血间充质干细胞血红素氧化酶1的表达,血红素氧化酶1可明显提高脐血间质干细胞梗死心肌移植后的细胞存活率。BACKGROUND： The heme oxygenase-1 has a strong endogenous antioxidant role, and can reduce inflammation and play a protecting role for cells. OBJECTIVE： To study the effect of heme oxygenase-1 on the survival rate of canine cord-derived mesenchymal stem cells transplanted in infarcted hearts. METHODS： Cord blood was obtained, and mononuclear cells were isolated by density gradient centrifugation and amplified by adherent culture. Mesenchymal stem cells of the fourth generation were treated by hemin for 24 hours, then the expression of heme oxygenase-1 enzyme was detected by immunohistochemistry. The fourth generation cells labeled by the LacZ reporter gene were transplanted into infracted area of hearts. RESULTS AND CONCLUSION： The heme oxygenase-1 expression of canine cord-derived mesenchymal stem cells following pretreated with hemin was significantly increased for more than 14 days. The cells transplanted into the infarcted area could survive for a long time. Image analysis showed that the 4, 8 weeks after transplantation, cell viability of the experimental group was significantly higher than control group. Preliminary results suggest that over-expression of heme oxygenase-1 can significantly increase the survival of cord-derived mesenchymal stem cells after transplanted into myocardial infarction hearts.

关 键 词：血红素氧化酶1 脐血 间充质干细胞 细胞移植 存活率 

分 类 号：R394.2[医药卫生—医学遗传学]