大鼠咬合创伤早期牙槽骨基因差异表达的初步探讨  

The primary study on the gene expression profiles of alveolar bone with traumatic occlusion in early stage in rats

作　　者：万浩元[1] 孙惠强[1,2] 商思霞[1] 李欣[1] 

机构地区：[1]山东大学口腔医学院 [2]山东省口腔生物医学重点实验室,济南250012

出　　处：《华西口腔医学杂志》2012年第2期183-186,共4页West China Journal of Stomatology

基　　金：山东省自然科学基金资助项目(ZR2010HM035);山东省医药卫生科技发展计划基金资助项目(2011WSB19002)

摘　　要：目的探讨咬合创伤早期牙槽骨吸收基因表达差异及相关信号通路。方法通过在大鼠左侧上颌第一磨牙粘接钢丝建立同侧下颌咬合创伤实验动物模型,24 h后局部麻醉下分离大鼠双侧下颌牙槽骨组织,提取总RNA进行包括27 000个基因的全功能组表达谱基因芯片检测,对差异表达基因进行Pathway和GO分析,并采用实时定量聚合酶链反应对芯片结果进行验证。结果芯片结果显示,差异基因共有586个,其中表达增强166个,表达降低420个,Pathway分析涉及106个不同的信号通路,GO分析主要涉及270个不同的功能分类。结论咬合创伤24 h牙槽骨已开始出现骨平衡变化,该期主要表现为成骨细胞和成骨活动抑制,破骨细胞发生及破骨现象增强尚未显现。Objective To study the gene expression profiles of traumatic occlusion in early stage with the animal model of rats.Methods The occlusal surface of the upper left first molar of rat was raised by placing a stainless steel wire to induce occlusal trauma in the lower left first molar.After 24 hours,the alveolar bone tissue of the first molars at the both sides of rats＇ lower jaws were taken out under anesthesia.The different expressive genes were shown by genome-wide microarray,which comprises about 27 000 genes and analyzed the different expressive genes with Path-way and GO analysis,finally the results of the microarray were examined by real time-polymerase chain reaction（RTPCR）.Results In the results of the study,586 different expressions were found,of which the expressions of 166 genes increased and 420 genes decreased.106 different pathways were involved with Pathway analysis and 270 different functional classification related to GO analysis.Conclusion The balance of the lower alveolar bone is destroyed after 24 hours of traumatic occlusion.At early phase of the occlusal trauma,osteogenesis and bone formation in alveolar bone are inhibited,yet osteoblast genesis and bone resorption are not significant.

关 键 词：咬合创伤 骨吸收 基因芯片 成骨细胞 破骨细胞 

分 类 号：R782[医药卫生—口腔医学]