CdS量子点的酿酒酵母仿生合成及光谱表征  

Biosynthesis of CdS Quantum Dots in Saccharomyces Cerevisiae and Spectroscopic Characterization

作　　者：黄淮青[1] 何明欣[1] 王文星[1] 刘金玲[1] 密丛丛[1] 徐淑坤[1] 

机构地区：[1]东北大学化学系,辽宁沈阳110819

出　　处：《光谱学与光谱分析》2012年第4期1090-1093,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(28075011)资助

摘　　要：以酿酒酵母为载体,常温下利用仿生法成功合成了CdS量子点。荧光发射光谱、紫外吸收光谱以及荧光显微镜照片证明,该方法合成的CdS量子点的荧光发射峰位置在443nm,在紫外灯下能发蓝绿色荧光。透射电子显微镜(TEM)表征结果表明,该仿生法合成的CdS量子点为六方纤锌矿结构。以荧光发射和紫外吸收光谱为性能指标,考察了酿酒酵母生长时期、Cd2+的反应浓度以及反应时间等条件对合成CdS量子点的影响。当酿酒酵母处于生长稳定期初期时,与浓度为0.5mmol.L-1的Cd2+共培养24h后所合成的CdS量子点荧光最强。实验中观察到,换液培养可有效提高酿酒酵母合成CdS量子点的产量。In the present work, CdS quantum dots （QDs） were successfully biosynthesized at room temperature by using saccharomyces cerevisiae yeast as a carrier. Fluorescence emission spectra, ultraviolet-visible （UV/Vis） absorption spectra, and inverted fluorescence microscope images confirmed that saccharomyces cerevisiae can be used to biosynthesize CdS QDs. The as-prepared CdS QDs show the fluorescence emission peak at 443 nm and emit blue-green fluorescence under UV light （with excitation at 365 nm）. Transmission electron microscopy （TEM） was applied to characterize the as-prepared CdS QDs and the TEM results showed that the as-prepared CdS QDs had the structure of hexagonal wurtzite. Fluorescence emission spectrum and UV/ Vis absorption spectrum were used as the performance indicatiors to study the effects of saeeharomyces cerevisiae yeast incubation times, reactant Cdz＋ concentrations and reaction times on CdS QDs synthesis. Saccharornyces cerevisiae yeast grown in early stable phase can get the highest fluorescence intensity of CdS QDs when they were co-cultured with 0.5 mmol ~ L 1 of Cdz＋ with 24 h incubation time. Furthermore, much more CdS QDs can be obtained by changing the culture medium during the synthesis process.

关 键 词：CDS 量子点 酿酒酵母 仿生合成 

分 类 号：O657[理学—分析化学]