花生黄曲霉抗性与SR和α-微管蛋白相关性  

Relationship between SR,α-Tubulin and Resistance to Aspergillus Flavas in Peanut

作　　者：严海燕[1] 宗成志[1] 翟刚[1] 马生财[1] 单世华[2] 

机构地区：[1]中南民族大学生命科学学院,武汉430074 [2]山东省花生研究所,青岛266100

出　　处：《中国生物工程杂志》2012年第3期32-38,共7页China Biotechnology

基　　金：山东省花生所合作资助项目(HZY05013)

摘　　要：信号识别颗粒受体在分泌性蛋白合成和分泌过程中起重要作用。微管蛋白对细胞内各种生命活动都是必需的。将数据库中花生抗黄曲霉和敏感品种的两类α-微管蛋白α和α7和SR蛋白进行序列比较分析发现,在种子发育早期5,α-微管蛋白在敏感品种各有三条EST,抗性品种各有一条EST,晚期7只有抗性品种各有一条EST。SR只在抗性品种的6、7时期各有一条EST,敏感品种中没有。用荧光定量PCR方法对抗性品种KB153与敏感品种JH1012发育中不同时期的果实和部位进行差异表达分析,结果表明在果实发育早期的小果期,SR、α-微管蛋白α和α7在抗性品种中都显著上调表达,说明SR介导的内质网蛋白质运输途径与黄曲霉抗性有关。SR基因在抗性品种的子叶中的表达也上调,这与抗性品种中一些贮藏蛋白含量尤其是蛋白酶抑制剂高于敏感品种的现象一致。Signal recognition particle receptor（SR） is important in secretive protein synthesis and secretion process.Tubulin is essential in various life process inside cells.Comparison between proteins encoded by ESTs of α-tubulins and SR in GenBank database of Aspergillus Flavas resistance（C20R） and sensitive（TFR） cultivars reveals that for two types of α-tubulins α and α7,there are 3 ESTs each in TFR early seed developmental stage 5,there is only one EST each in C20R seed developmental stage 5 and 7.For SR,only in C20R seed developmental stage 6 and 7,there is one EST in each stage,none in any seed developmental stage of TFR.QPCR assay on expression of α-tubulins and SR in different organ and developmental stage of peanut fruit of Aspergillus Flavas resistance cultivar KB153 and sensitive cultivar JH1012 indicates that in small fruit stage of fruit early developmental stage,SR and two types of α-tubulins all up-regulated in Aspergillus Flavas resistance cultivar KB153 than sensitive cultivar JH1012.This suggests that protein transport via ER intermediated by SR is related to Aspergillus Flavas resistance.SR is also up-related in cotyledons of Aspergillus Flavas resistance cultivar KB153.This is corresponds to the higher content of storage protein including proteinase inhibitor in Aspergillus Flavas resistance cultivars.

关 键 词：黄曲霉 花生 Α-微管蛋白 果实发育早期 SR 

分 类 号：Q5[生物学—生物化学]