免疫固定电泳法检测尿本-周蛋白的临床应用  

Clinical application of immunofixation electrophoresis detection of urine Bence-Jones protein

作　　者：曾祝伦[1] 张司兰 熊曼[1] 

机构地区：[1]重庆医科大学附属第二医院检验科,400010 [2]重庆市璧山县人民医院心血管内科,402760

出　　处：《重庆医学》2012年第8期762-763,共2页Chongqing medicine

摘　　要：目的探讨免疫固定电泳检测尿本-周蛋白(BJP)的临床价值。方法采用免疫固定电泳及热沉淀反应法检测尿液BJP,并比较二者的灵敏度和特异性。结果 140例晨尿样本用免疫固定电泳检测,检出尿BJP阳性42例,其中IgGκ型BJP 26例,IgMλ型BJP 16例;用热沉淀反应法检测,检出尿BJP阳性24例。76例十二烷基硫酸钠-琼脂糖凝胶电泳(SDS-AGE)提示尿蛋白异常的样本经免疫固定电泳的BJP检出率为55.3%,热沉淀反应法的检出率为31.6%。结论免疫固定电泳检测尿BJP具有较高的灵敏度和特异性。Objective To investigate the clinical value of immunofixation electrophoresis detection of urine Bence-Jones protein （BJP）. Methods Immunofixation electrophoresis and thermal precipitation reaction were utilized to detect urine BJP,and their sensitivity and specificity were compared. Results 140 samples of morning urine were detected by immunofixation electrophoresis,42 samples of them were found to be BJP positive,with 26 cases of IgG k type BJP and 16 cases of IgM λ, type BJP. The same 140 samples were detected by thermal precipitation reaction,and 24 samples of them were found to be BJP positive. 76 samples with abnormal urine protein indicated by sodium dodecyl sulfate-agarose gel electrophoresis（SDS-AGE） were subjected to immunofixation eleetrophoresis and thermal precipitation reaction, respectively, and their BJP detection rate was 55.3% and 31.6 %, respectively. Conclusion Immunofixation electrophoresis possesses higher sensitivity and specificity for urine BJP detection.

关 键 词：本周蛋白 免疫电泳 免疫球蛋白类 

分 类 号：R733.3[医药卫生—肿瘤]