大鼠脑卒中痉挛性瘫痪模型制备方法研究  

Study on Model Preparation Method of Stroke Spastic Paralysis in Rats

作　　者：陈乐乐[1] 岳增辉[1] 朱小姗[1] 

机构地区：[1]湖南中医药大学针灸推拿学院,湖南长沙410007

出　　处：《上海中医药大学学报》2012年第2期75-77,共3页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：湖南省高校创新平台开放基金资助项目(10K046);湖南省科技计划基金资助项目(2010JT4015);湖南省自然科学基金资助项目(11JJ6080);湖南省研究生科研创新项目(CX2011B344);湖南省经穴-脏腑相关重点研究室;针灸推拿学重点学科;针灸生物信息分析重点实验室建设资助项目

摘　　要：目的:探索制作稳定且成功率高的大鼠脑卒中痉挛性瘫痪模型。方法:分别运用文献报道的电损毁内囊法及线栓法制备大鼠脑卒中痉挛模型。结果:电损毁内囊法造模失败,且在改变内囊定位、减小电流量及刺激时间后动物死亡率仍然为100%;线栓法造模存活动物神经系统损伤症状评分、肌张力评分均符合要求,成活率为80%。结论:线栓法制作大鼠脑卒中痉挛性瘫痪模型较稳定,且成功率高,有一定的可行性。Objective： To explore the way to establish stable and successful rat models of stroke spastic paralysis.Methods： We established rat models of stroke spastic paralysis by electrolytic damage of capsula interna method and suture-occlusion method.Results： Establishing the models by electrolytic damage of capsula interna method failed,and the death rate was 100% after changed location of capsula interna,reduced electricity and stimulation time;the survived animals met the requirements of neurological deficit symptom and spasticity assessment,and the survival rate of the model prepared by suture-occlusion method was 80%.Conclusion： The rat model of stroke spastic paralysis prepared by suture-occlusion method is stable,practical and feasible.

关 键 词：脑卒中痉挛性瘫痪 线栓法 电损毁内囊法 动物模型 

分 类 号：R-332[医药卫生] R743.3