中国北方地区汉族人群血小板膜糖蛋白Ⅰbα VNTR多态性的分布与脑梗死患者CC基因型与阿司匹林敏感相关性分析  

Variable number of tandem repeats polymorphism of platelet glycoprotein Ib a in Chinese people and CC genotype with aspirin sensitivity in patients with cerebral infarction

作　　者：辛晓敏[1] 蒋丽鑫[1] 王瑶[2] 金英玉[1] 张云平[1] 

机构地区：[1]哈尔滨医科大学附属第一医院检验科,哈尔滨150001 [2]北京朝阳医院京西院区检验科

出　　处：《中国实验诊断学》2012年第3期481-483,共3页Chinese Journal of Laboratory Diagnosis

基　　金：黑龙江省教育厅科学技术研究项目资助(项目编号:11531141)

摘　　要：目的研究中国北方地区汉族人群血小板膜糖蛋白(GP)Ⅰbα数目可变串联重复(VNTR)多态性与阿司匹林抵抗(AR)的关系。方法用聚合酶链反应限制性片段长度多态性(PCR-RFLP)检测300例健康人及110脑梗死患者基因型。脑梗死患者服用阿司匹林(100mg/d)至少7d以上,用二磷酸腺苷(ADP)和花生四烯酸(AA)作诱导剂测定血小板聚集功能。结果中国北方地区汉族人群中只检测出GPⅠbα三种等位基因B、C、D,没有发现A基因型。阿司匹林敏感(AS)患者CC基因型比阿司匹林半抵抗(ASR)患者更多见。结论中国东北地区仅检测出B、C、D三种基因型。GPⅠb VNTR多态性CC基因型的出现对于低剂量阿司匹林抗血小板作用更为敏感。Objective To study the prevalence of variable number of tandem repeats（VNTR） polymorphism in platelet membrane glycoprotein（GP） Ib α in a Chinese Han population and to determine the relationship between VNTR polymorphisms and aspirin resistance.Methods Three hundred healthy individuals and 110 patients with cerebral infarction volunteered to participate in this study.The genotype status of all participants was determined by polymerase chain reaction-restriction fragment length polymorphism analysis.Platelet aggregation in patients with cerebral infarction receiving aspirin（100 mg/day） for at least 7 days,was measured by optical transmission aggregometry.Results Only three alleles of GPIb α,namely,B,C and D,were found.Type A was not found in the Chinese Han participants.Aspirin-sensitive patients were significantly more often of CC genotype than aspirin-semiresponders.Conclusion Only three types of alleles B,C and D were detected in the north-eastern region of China.The CC genotype of the VNTR polymorphism in GPIb appears to be more sensitive to the inhibitory action of low-dose aspirin.

关 键 词：阿司匹林抵抗 阿司匹林敏感 脑梗死 基因多态性 血小板膜糖蛋白 

分 类 号：R743.3[医药卫生—神经病学与精神病学]