经静脉注射脂质包裹液态氟碳纳米粒增效高强度聚焦超声治疗肿瘤的初步研究  

Preliminary Study of Increased Efficacy of High Intensity Focused Ulrasound Treating Tumors Using Lipids Encapsulated Liquid Fluorocarbon Nanoparticles by Intravenous Injection

作　　者：周洋[1] 郑元义[1] 沈红霞[1] 余进洪[1] 孙阳[1] 王志刚[1] 

机构地区：[1]重庆医科大学附属第二医院超声影像学研究所,重庆市400010

出　　处：《中国超声医学杂志》2012年第3期193-196,共4页Chinese Journal of Ultrasound in Medicine

基　　金：国家重点基础研究发展计划(973计划)(No.2011CB707900);国家自然科学基金(No.81071158,No.81130025)

摘　　要：目的观察经静脉注射脂质包裹液态氟碳纳米粒对高强度聚焦超声(HIFU)治疗肿瘤的增效作用,并探讨其增效机制。方法首先制备脂质包裹的液态氟碳纳米粒。然后选取建模成功后3周的荷VX2肝癌移植瘤的新西兰大白兔36只,随机等分为3组,用HIFU对兔肝肿瘤进行辐照。A组为对照组,即辐照前经兔耳缘静脉注射生理盐水,B组在辐照前注射液态氟碳纳米粒,30 s后用HIFU辐照,C组在辐照前24 h注射液态氟碳纳米粒。观察各组辐照区域视频灰度变化及肿瘤凝固性坏死情况。结果 B组和C组均见到辐照区域明显灰度变化,A组仅部分见到灰度变化,B、C组辐照前后灰度变化面积和变化值与A组有显著差异(P<0.05);3组兔肝肿瘤内均观察到明显的凝固性坏死,B、C组坏死的范围明显大于A组(P<0.05)。结论经静脉注射脂质包裹的液态氟碳纳米粒能够增强HIFU的疗效,此效应可能与纳米粒改变组织声环境、增加聚焦超声的空化作用有关,但具体作用机制有待进一步探讨。Objective To observe the increased efficacy of HIFU treating tumors using lipids encapsulated liquid fluorocarbon nanoparticles by intravenous injection and explore the synergistic mechanism. Methods Firstly, the lip- ids coated liquid fluorocarbon nanoparticles were prepared. Thirty-six New Zealand white rabbits borne VX2 tumors in liver for 3 weeks were randomly divided into three groups and underwent focused ultrasound irradiation. A group （the control group）, was irradiated 30 s after the injection of saline by the rabbit ear vein, B group was irradiated 30 s after the injection of liquid fluorocarbon nanoparticles, C group was irradiated 24 hours after the injection of liquid fluoro- carbon nanoparticles. The gray changes of irradiation areas and coagulation necrosis in tumors were observed. Results Obvious gray change can be seen in all of group B and C, but partly in group A. There were significant differences in gray change values and squares among group B, C and A（P~ 0.05）. Coagulative necrosis with round shape and clear boundary was observed in every group immediately after irradiation, and the range of necrosis in group B and C was much larger than that in group A （P^0.05）. Conclusions The lipids encapsulated liquid fluorocarbon nanoparticles by intravenous injection can increase the therapeutic efficacy of HIFU. The effect may be due to the nan- oparticles changed the sound environment of tissue, and increased the cavitation of focused ultrasound, but the exact mechanism needs further investigation.

关 键 词：纳米粒 液态氟碳 治疗 高强度聚焦超声 

分 类 号：R730.5[医药卫生—肿瘤]